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Genomic organization of the canine p53 gene and its mutational status in canine mammary neoplasia

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Abstract

To determine whether canine malignancies share common genetic lesions with their human counterparts, and are thus potentially interesting model systems in which to pose questions regarding tumor etiology and progression, we have elucidated the entire exon/intron structure of the canine p53 gene. A search for p53 gene abnormalities in mammary tumor tissue was undertaken utilizing single strand conformation polymorphism analysis. Mutations were detected in exons 4, 5, 6, and 7 of the p53 gene and consisted of nonsense, splicing, and frameshift mutations. None of 11 benign tumors and 6 of 40 primary carcinomas (15%) were found to harbor subtle p53 mutations. In 14 carcinomas examined the results in primary tumors and metastases were the same. These findings implicate involvement of this gene in the genesis of some malignant canine tumors, in a fashion similar to their human counterparts.

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Authors and Affiliations

  1. Department of Biochemistry, Canada

    Lee Lee Chu, Julius M.C. Kong, Majid Ghahremani, Martin Schmeing & Jerry Pelletier

  2. Department of Clinical Sciences of Companion Animals, Netherlands

    Gerard R. Rutteman & Wim Misdorp

  3. Department of Pathology, Faculty of Veterinary Medicine, University of Utrecht, Netherlands

    Wim Misdorp

  4. Department of Pathology, Faculty of Veterinary Medicine, University of Utrecht, Netherlands

    Evert van Garderen

  5. Department of Oncology, McGill University, Montréal, Québec, Canada

    Jerry Pelletier

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  1. Lee Lee Chu
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Chu, L.L., Rutteman, G.R., Kong, J.M. et al. Genomic organization of the canine p53 gene and its mutational status in canine mammary neoplasia. Breast Cancer Res Treat 50, 11–25 (1998). https://doi.org/10.1023/A:1006010526813

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