Abstract
The characterization of a cDNA clone encoding non-specific lipid transfer protein (PvLTP, formerly named PVR3) in the roots of bean seedlings has been previously reported. In this study, we examined the temporal and spatial accumulation of PvLTP mRNA and the effect of the auxin naphthaleneacetic acid (NAA) on the accumulation of PvLTP mRNA during root development. In situ hybridization showed that accumulation of PvLTP mRNA is highly tissue-specific. Accumulation was detected in the cortical tissue, but not in other tissues of root, including the quiescent center and root cap. Within the cortical tissue, accumulation of PvLTP mRNA was developmentally regulated; accumulation of PvLTP mRNA was high in the cortical tissue of the proximal and ground meristem and declined as cortical tissue developed further. Since the appropriate distribution of auxin is an important factor responsible for the maintenance of root meristem organization. We examined effect of auxin on the accumulation of PvLTP mRNA in relation to the development of cortical tissue. In bean seedlings grown on medium supplemented with 5 μM NAA, morphological alternations, including radial root expansion and abnormal tissue organization in the root apical meristem, were observed. Only faint accumulation signals of PvLTP mRNA were observed in the cortical tissue of proximal meristem region, indicating that cortical tissue development was repressed by exogenous NAA. However, our results suggest that the change in accumulation of PvLTP mRNA is not direct regulatory effect but reflective effect of altered development of cortical tissue that was induced by exogenous NAA. The temporal and spatial accumulation of PvLTP mRNA indicates that PvLTP is a useful marker for the development of cortical tissue in the root tip in bean seedlings.
Similar content being viewed by others
References
Barlow PW: The concept of the stem cell in the context of plant growth and development. In: Lord BI, Potten CS, Cole RJ (eds) Stem Cells and Tissue Homeostasis pp. 89–113. Cambridge University Press, Cambridge, UK (1978).
Blakesley D, Weston GD, Hall JF: The role of endogenous auxin in root initiation. I. Evidence from studies on auxin application, and analysis of endogenous levels. Plant Growth Regul 10: 341–353 (1991).
Burstr HG: Influence of the tonic effect of gravitation and auxin on cell elongation and polarity in root. Am J Bot 56: 697–684 (1969).
Canevascini S, Caderas D, Mandel T, Fleming AJ, Dupuis I, Kuhlemeier C: Tissue-specific expression and promoter analysis of the tobacco ltp1 gene. Plant Physiol 112: 513–524 (1996).
Chasan R: Lipid transfer proteins: moving molecules? Plant Cell 3: 842–843 (1991).
Choi D-W, Song JY, Kwon YM, Kim S-G: Characterization of cDNA encoding a proline-rich 14 kDa protein in developing cortical cells of the roots of bean (Phaseolus vulgaris) seedlings. Plant Mol Biol 30: 973–982 (1996).
Choi D-W, Song, JY, Oh M-H, Lee JS, Moon J, Suh SW, Kim S-G: Isolation of root-specific cDNA encoding a ns-LTP-like protein from the roots of bean (Phaseolus vulgaris) seedlings. Plant Mol Biol 30: 1059–1066 (1996).
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159 (1987).
Cordoba F, Gonzales-Reyez JA: Ascorbate and plant cell growth. J Bioenerget Biomembr 26: 399–406 (1994).
Cox KH, Goldberg RB: Analysis of plant gene expression. In: Shaw CH (ed) Plant Molecular Biology: A Practical Approach, pp. 1–35. IRL Press, Oxford (1988).
Esau K: Pflanzenanatomie. Gustav Fischer Verlag, Stuttgart (1969).
Esau K: Anatomy of Seed Plant, 2nd ed. John Wiley, New York (1977).
Evans ML, Ishikawa H, Estelle MA: Responses of Arabidopsis roots to auxin studied with high temporal resolution: Comparison of wild type and auxin-response mutants. Planta 194: 215–222 (1994).
Fleming AJ, Mandel T, Hofmann S, Sterk P, de Vries SC, Kuhlemeier C: Expression pattern of a tobacco lipid transfer protein gene within the shoot apex. Plant J 2: 855–862 (1992).
Kader J-C: Lipid-transfer proteins in plants. Annu Rev Plant Physiol Plant Mol Biol 47: 627–654 (1996).
Kader J-C: Lipid-transfer proteins: a puzzling family of plant proteins. Trends Plant Sci 2: 66–70 (1997).
Kerk NM, Feldman LJ: A biochemical model for the initiation and maintenance of the quiescent center: implications for organization of root meristems. Development 121: 2825–2833 (1995).
King JJ, Stimart DP, Fisher RH, Bleecker AB: A mutation altering auxin homeostasis and plant morphology in Arabidopsis. Plant Cell 7: 2023–2037 (1995).
Krause A, Sigrist CJA, Dehning I, Sommer H: Accumulation of transcripts encoding a lipid transfer-like protein during deformation of nodulation-competent Vigna unguiculata root hairs. Mol Plant-Microbe Interact 7: 411–418 (1994).
Molina A, Segura A, Garcia-Olmedo F: Lipid transfer proteins (ns-LTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. FEBS Lett 316: 119–122 (1993).
Nielsen KK, Nielsen JE, Madrid SM, Mikkelsen JD: New antifungal proteins from sugar beat (Beta vulgaris L.) showing homology to non-specific lipid transfer proteins. Plant Mol Biol 31: 539–552 (1996).
Pilet P, Saugy M: Effect of applied and endogenous indol-3-ylacetic acid on maize root growth. Planta 164: 254–258 (1985).
Pyee J, Kolattukudy PE: The gene for the major cuticular waxassociated protein and three homologous genes from broccoli (Brassica oleracea) and their expression patterns. Plant J 7: 49–59 (1995).
Sambrook J, Fritsch EE, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sossountzov L, Ruiz-Avila L, Vignols F, Jolliot A, Arondel V, Tchang F, Grosbois M, Guerbette F, Miginiac E, Delseny M, Puigdomenech P, Kader J-C: Spatial and temporal expression of a maize lipid transfer protein gene. Plant Cell 3: 923–933 (1991).
Steeves TA, Sussex IM: Patterns in Plant Development, 2nd ed. Cambridge University Press, Cambridge, UK (1989).
Sterk P, Booij H, Scheleekens GA, van Kammen A, de Vries SC: Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell 3: 907–921 (1991).
Tchang F, This, P, Stiefel V, Arondel V, Morch M-D, Pages M, Puigdomenech P, Grellet F, Delseny M, Bouillon P, Huet J-C, Guerbette F, Beauvais-Cante F, Duranton H, Pernollet JC, Kader J-C: Phospholipid transfer protein: full-length cDNA and amino acid sequence in maize. J Biol Chem 263: 16849–16855 (1988).
Terras FRG, Goderis IJ, Van Leuven F, Vanderleyden J, Cammue BPA, Broekaert WF: In vitro antifungal activity of a radish (Raphanus sativus L.) seed protein homologous to nonspecific lipid transfer proteins. Plant Physiol 100: 1055–1058 (1992).
Thoma, S, Hecht U, Kippers A, Botella J, de Vries S, Somerville C: Tissue-specific expression of a gene encoding a cell wall-localized lipid transfer protein from Arabidopsis. Plant Physiol 105: 35–45 (1994).
Thoma S, Kaneko Y, Somerville C: A non-specific lipid transfer protein from Arabidopsis is a cell wall protein. Plant J 3: 427–436 (1993).
Torres-Schumann S, Godoy JA, Pintor-Toro JA: A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants. Plant Mol Biol 18: 749–757 (1992).
Vignols F, Lund G, Pammi S, Tremousaygue D, Grellet F, Kader J-C, Puigdomenech P and Delseny M: Characterization of rice gene coding for a lipid transfer protein. Gene 142: 265–270 (1994).
Webster BD, Radin JW: Growth and development of cultured radish roots. Am J Bot 59: 744–751 (1972).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Song, J.Y., Choi, DW., Lee, J.S. et al. Cortical tissue-specific accumulation of the root-specific ns-LTP transcripts in the bean (Phaseolus vulgaris) seedlings. Plant Mol Biol 38, 735–742 (1998). https://doi.org/10.1023/A:1006008117795
Issue Date:
DOI: https://doi.org/10.1023/A:1006008117795