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Metabolism of zeatin riboside in a hormone autonomous genetic tumour line of tobacco

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Abstract

The metabolic fate of externally applied [3H]-zeatin riboside ([9R]Z) was studied in a cultured genetic tumour line of Nicotiana glauca (Grah.) × N. langsdorffii (Weinm.), which grows on auxin and cytokinin free medium. Metabolism by 3.5-week-old tissues showed enhanced stability of supplied [9R]Z; unmetabolized [9R]Z accounted for 48.7 and 37.5% of extracted radioactivity following 8 and 24 h incubation, respectively; tissues of different ages (1–10 weeks following subculture) also indicated high cytokinin stability following 8 h incubation (unmetabolized [9R]Z accounted for 32.5–53.0% of extracted radioactivity). All analyses were performed by thin layer chromatography (TLC) and the results subsequently confirmed by high performance liquid chromatography (HPLC). Side-chain cleavage and modification of the purine ring were the major forms of metabolism; metabolites with an intact cytokinin moiety included zeatin (Z), [9R]Z nucleotides and glucosyl derivatives. Detailed analysis of metabolites carried out in the experiments using 3.5-week-old tissues indicated that both dihydro-derivatives as well as cis isomers of Z and [9R]Z were not formed. Adenine, adenosine and its nucleotide(s) were the main degradative metabolites; in 3.5-week-old tissues these metabolites accounted for about 5.9 and 7.8% (of 3H extracted) following 8 and 24 h incubation, respectively. In tissues of different ages (1–10 weeks following subculture), these metabolites accounted for about 7.6–22.9% of the extracted 3H. Some metabolites (zeatin, adenine and adenosine) were also detected in the staled incubation media. The observed high [9R]Z stability in this tissue may reflect low levels of cytokinin oxidase activity and/or some form of compartmentation.

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Author notes

  1. Shyamal K. Nandi & Lok Man S. Palni

    Present address: Department of Environmental Physiology & Biotechnology, G.B. Pant Institute of Himalayan Environment & Development, Kosi-Katarmal, Almora, 263 643, U.P., India

Authors and Affiliations

  1. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, G.P.O. Box 475, Canberra City, ACT 2601, Australia

    Shyamal K. Nandi & Lok Man S. Palni

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  1. Shyamal K. Nandi
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  2. Lok Man S. Palni
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Nandi, S.K., Palni, L.M.S. Metabolism of zeatin riboside in a hormone autonomous genetic tumour line of tobacco. Plant Growth Regulation 23, 159–166 (1997). https://doi.org/10.1023/A:1005985331661

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