Abstract
Tomato and potato leucine aminopeptidase (LAP) mRNAs are induced in response to mechanical wounding and the wound signal molecules, ABA and jasmonic acid. Here, we report the isolation of two LAP genes, LAP17.1A and LAP17.2, from tomato. Functional analysis in transgenic tomato and potato plants show that fusions of the corresponding 5′ non-coding regions to the gusA gene are constitutively expressed in flowers and induced in leaves upon wounding or by treatment with methyl jasmonate (MeJA). Comparison of the 5′ non-coding regions of the two genes revealed a region from –317 to –3 relative to the ATG, which is strongly conserved in both promoters. This 0.3 kb proximal promoter fragment is sufficient to direct flower-specific and MeJA-inducible GUS activity in transgenic potato plants, and thus contains a MeJA-responsive element that mediates induction by MeJA. Dimeric TGACG motifs or G-box elements similar to those found in other MeJA-inducible genes are not observed in this region, which suggests that a different DNA sequence is involved in MeJA induction of the LAP genes.
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Albrecht T, Kehlen A, Stahl K, Knöfel HD, Sembdner G, Weiler EW: Quantification of rapid, transient increases in jasmonic acid in wounded plants using a monoclonal antibody. Planta 191: 86–94 (1993).
Amasino RM: Acceleration of nucleic acid hybridization rate by polyethyleneglycol. Anal Biochem 152: 304–307 (1986).
Bell E, Mullet JE: Lipoxygenase gene expression is modulated in plants by water deficit, wounding, and methyl jasmonate. Mol Gen Genet 230: 456–462 (1991).
Creelman RA, Tierney ML, Mullet JE: Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypoctyls and modulate wound gene expression. Proc Natl Acad Sci USA 89: 4938–4941(1992).
Creelman RA, Mullet JE: Jasmonic acid distribution and action in plants. Regulation during development and response to biotic and abiotic stress. Proc Natl Acad Sci USA 92: 4114–4119 (1995).
Dellaporta SL, Wood J, Hicks JB: A plant DNA minipreparation: Version II. Plant Mol Biol Rep 1: 19–21 (1983).
Genetics Computer Group: Program manual for the Wisconsin package, version 8 (1994).
Dittrich H, Kutchan T, Zenk MH: The jasmonate precursor, 12oxophytodienoic acid, induces phytoalexin synthesis in Petroselinum hortense cell cultures. FEBS Lett 309: 33–36 (1992).
Doares SH, Narvaez Vazquez J, Conconi A, Ryan C: Salicylic acid inhibits synthesis of proteinase inhibitors in tomato leaves induced by sytemin and jasmonic acid. Plant Physiol 108: 1741–1746 (1995)
Farmer EE, Ryan CA: Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proc Natl Acad Sci USA 87: 7713–7716 (1990).
Farmer EE: Fatty acid signalling in plants and their associated microorganisms. Plant Mol Biol 26: 1423–1437 (1994).
Foster R, Izawa T, Chua NH: Plant bZIP proteins gather at ACGT elements. FASEB J 8: 192–200 (1994).
Green TR, Ryan CA: Wound-induced proteinase inhibitor in plant leaves. A possible defence mechanism against insects. Science 175: 776–777 (1972).
Gu YQ, Pautot V, Holzer FM, Walling LL: A complex array of proteins related to the multimeric leucine aminopeptidase of tomato. Plant Physiol 110: 1257–1266 (1996).
Gundlach H, Müller MJ, Kutchan TM, Zenk MH: Jasmonic acid is a signal transducer in elicitorinduced plant cell cultures. Proc Natl Acad Sci USA 89: 2389–2393 (1992).
Hildmann T, Ebneth M, Peña-Cortés H, Sánchez-Serrano JJ, Willmitzer L, Prat S: General roles of jasmonic and abscisic acids in gene activation as a result of mechanical wounding. Plant Cell 4: 1157–1170 (1992).
Höfgen R, Willmitzer L: Storage of competent cells for Agrobacterium transformation. Nucl Acids Res 16: 9877 (1988).
Jefferson RA: Assaying chimeric genes in plants: the GUSgene fusion system. Plant Mol Biol Rep 5: 387–405 (1987).
Kernan A, Thornburg RW: Auxin levels regulate the expression of a woundinducible proteinase inhibitor IIchloramphenicol acetyl transferase gene fusion in vitro and in vivo. Plant Physiol 91: 73–78 (1989)
Kim SR, Choi JL, Costa MA, An G: Identification of Gbox sequence as an essential element formethyl jasmonate response of potato proteinase inhibitor II promoter. Plant Physiol 99: 627–631 (1992).
Koornneef M, Hanhart C, Jongsma M, Toma I, Weide R, Zabel P, Hille J: Breeding of a tomato genotype readily accesible to genetic manipulation. Plant Sci 45: 201–208 (1986).
Koornneef M, Bade J, Hanhart C, Horsman K, Schel J, Soppe W, Verkerk R, Zabel P: Characterization and mapping of a gene controlling shoot regeneration in tomato. Plant J 3: 131–141 (1993).
Liu XJ, Prat S, Willmitzer L, Frommer WB: Cis regulatory elements directing tuberspecific and sucroseinducible expression of a chimeric class I patatin promoter/GUSgene fusion. Mol Gen Genet 223: 401–406 (1990).
Logemann J, Schell J, Willmitzer L: Improved method for the isolation of RNA from plant tissue. Anal Biochem 163: 16–20 (1987).
Mason HS, DeWald DB, Mullet JE: Identification of a methyl jasmonateresponsive domain in the soybean vspB promoter. Plant Cell 5: 241–251 (1993).
McGurl B, Pearce G, Orozco Cárdenas M, Ryan CA: Structure, expression, and antisense inhibition of the systemin precursor gene. Science 255: 1570–1573 (1992).
Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497 (1962).
Pautot V, Holzer FM, Reisch B, Walling LL: Leucine aminopeptidase: an inducible component of the defense response in Lycopersicon esculentum (tomato). Proc Natl Acad Sci USA 90: 9906–9910 (1993).
Pearce G, Strydom D, Johnson S, Ryan CA: A polypeptide from tomato leaves activates the expression of proteinase inhibitor genes. Science 253: 895–897 (1991).
PeñaCortés H, SánchezSerrano JJ, Mertens R, Willmitzer L, Prat S: Abscisic acid is involved in the woundinduced expression of the proteinase inhibitor II gene in potato and tomato. Proc Natl Acad Sci USA 86: 9851–9855 (1989).
PeñaCortés H, Albrecht T, Prat S, Weiler EW, Willmitzer L: Aspirin prevents woundinduced gene expression in tomato leaves by blocking jasmonic acid biosynthesis. Planta 191: 123–128 (1993).
Rocha-Sosa M, Sonnewald U, Frommer W, Stratmann M, Schell J, Willmitzer L: Both developmental and metabolic signals activate the promoter of a class I patatin gene. EMBO J 8: 23–29 (1989).
Rouster J, Leah R, Mundy J, Cameron-Vills V: Identification of a methyl jasmonateresponsive region in the promoter of a lipoxygenase 1 gene expressed in barley grain. Plant J 11: 513–523 (1997).
Samach A, Broday L, Hareven D, Lifschitz E: Expression of an amino acid biosynthesis gene in tomato flowers: developmental upregulation and MeJA response are parenchymaspecific and mutually compatible. Plant J 8: 391–406 (1995).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Williams ME, Foster R, Chua NH: Sequences flanking the hexameric Gbox core CACGTG affect the specificity of protein binding. Plant Cell 4: 485–496 (1992).
Xiang C, Miao ZH, Lam E: Coordinated activation of as1type elements and a tobacco glutathione Stransferase gene by auxins, salicylic acid, methyl jasmonate and hydrogen peroxide. Plant Mol Biol 32: 415–426 (1996).
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Ruíz-Rivero, O.J., Prat, S. A –308 deletion of the tomato LAP promoters is able to direct flower-specific and MeJA-induced expression in transgenic plants. Plant Mol Biol 36, 639–648 (1998). https://doi.org/10.1023/A:1005980028203
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DOI: https://doi.org/10.1023/A:1005980028203