Abstract
The effects of 1,1-dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS) on the in vitro activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase and ACC synthase isolated from senescing carnation petals were investigated. In contrast to a previous proposal, DPSS at 1 mM did not inhibit the in vitro activity of ACC oxidase. It was confirmed that DPSS does not inhibit ACC synthase activity. DPSS probably does not exert its inhibitory action on ethylene production by a direct action on ACC oxidase and ACC synthase, but by some unknown action.
Similar content being viewed by others
References
Bouzayen M, Felix G, Latche A, Pech J-C and Boller T (1991) Iron: an essential cofactor for the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene. Planta 184: 244–247
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254
Fernandez-Maculet JC and Yang SF (1992) Extraction and partial characterization of the ethylene-forming enzyme from apple fruit. Plant Physiol 99: 751–754
Kosugi Y, Oyamada N, Satoh S, Yoshioka T, Onodera E and Yamada Y (1997) Inhibition by 1-aminocyclobutane-1-carboxylate of the activity of 1-aminocyclopropane-1-carboxylate oxidase obtained from senescing petals of carnation (Dianthus caryophyllus L.) flowers. Plant Cell Physiol 38: 312–318
Lizada MCC and Yang SF (1979) A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Anal Biochem 100: 142–147
McGarvey DJ and Christoffersen RE (1992) Characterization and kinetic parameters of ethylene-forming enzyme from avocado fruit. J Biol Chem 367: 5964–5967
Midoh N, Saijou Y, Matsumoto K and Iwata M (1996) Effects of 1,1-dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS) on carnation flower longevity. Plant Growth Regul 20: 195–199
Nakajima N and Imaseki H (1986) Purification and properties of 1-aminocyclopropane-1-carboxylate synthase of mesocarp of Cucurbita maxima Duch. fruits. Plant Cell Physiol 27: 969–980
Satoh S and Esashi Y (1980) α-Aminoisobutyric acid: A probable competitive inhibitor of conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene. Plant Cell Physiol 21: 939–949
Satoh S, Mori H and Imaseki H (1993) Monomeric and dimeric forms and the mechanism-based inactivation of 1-aminocyclopropane-1-carboxylate synthase. Plant Cell Physiol 34: 753–760
Satoh S, Yoshioka T and Kosugi Y (1995) Characteristics of 1-aminocyclopropane-1-carboxylate synthase isolated from senescing carnation flower petals. Acta Hortic 394: 297–304
Smith JJ, Ververidis P and John P (1992) Characterization of the ethylene-forming enzyme partially purified from melon. Phytochemistry 31: 1485–1494
Toshima H, Niwayama Y, Nagata H, Greulich F and Ichihara A (1993) Inhibitory effects of coronamic acid derivatives on senescence in cut carnation flowers. Biosci Biotech Biochem 57: 1394–1395
Yang SF and Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35: 155–189
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Satoh, S., Oyamada, N., Yoshioka, T. et al. 1,1-Dimethyl-4-(phenylsulfonyl)semicarbazide (DPSS) does not inhibit the in vitro activities of 1-aminocyclopropane-1-carboxylate (ACC) oxidase and ACC synthase obtained from senescing carnation Dianthus caryophyllus L.) petals. Plant Growth Regulation 23, 191–193 (1997). https://doi.org/10.1023/A:1005973220410
Issue Date:
DOI: https://doi.org/10.1023/A:1005973220410