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Isolation and characterization of a functional A-type cyclin from maize

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Abstract

Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic cyclins. Complementation studies revealed that cycZm2w was able to rescue a budding yeast cyclin-deficient mutant (BF305–15d#21). As expected, cycZm2w is expressed in organs of the maize plant that possess meristematic activity, but is especially prominent in the proliferating regions of the root apex.

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  1. Department of Plant Biology, University of Maryland, College Park, MD, 20742, USA

    Wen-Ling Hsieh & Stephen M. Wolniak

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  1. Wen-Ling Hsieh
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Hsieh, WL., Wolniak, S.M. Isolation and characterization of a functional A-type cyclin from maize. Plant Mol Biol 37, 121–129 (1998). https://doi.org/10.1023/A:1005968901298

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