Abstract
Four different cDNA clones encoding protein synthesis elongation factor 1A, eEF1A, were isolated from rice (Oryza sativa L.). The genes encoded by these cDNAs were designated rice elongation factor 1A genes refa1, refa2, refa3 and refa4. The genes encoded identical eEF-1A polypeptides and shared high amino acid identity with eEF1A of other eukaryotes. Southern blot analysis suggested that some of these refa genes may be organized in a cluster on the same chromosome within a short distance. PCR analysis of rice genomic DNA showed that refa1 and refa4, and refa3 and refa2 are in neighboring locations on the rice genome. The mRNAs of the four refa genes accumulated to nearly equal levels in a variety of tissues and at different stages of growth. Suspension-cultured cells were the most abundant in refa mRNAs. Dormant seeds contained a small amount of the four refa mRNAs. Transcript accumulation was highly induced after seed germination, and the same expression levels were maintained even in old leaf blades of mature plants.
Similar content being viewed by others
References
Aguilar F, Montandon P-E, Stutz E: Two genes encoding the soybean translation elongation factor eEF-1α are transcribed in seedling leaves. Plant Mol Biol 17: 351–360 (1991).
Arai K, Clark BFC, Duffy L, Jones MD, Kaziro Y, Laursen RA, L'Italien J, Miller DL, Nagarkatti S, Nakamura S, Nielsen KM, Petersen TE, Takahashi K, Wade M: Primary structure of the elongation factor Tu from Escherichia coli. Proc Natl Acad Sci USA 77: 1326–1330 (1980).
Arcari P, Gallo M, Ianniciello G, Russo AD, Bocchini V: Primary structure of the elongation factor 1 α in Sulfolobus solfataricus. Nucl Acids Res 21: 1666 (1993).
Axelos M, Bardet C, Liboz T, Thai ALV, Curie C, Lescure B: The gene family encoding the Arabidopsis thaliana translation elongation factor EF-1α: molecular cloning, characterization and expression. Mol Gen Genet 219: 106–112 (1989).
Berberich T, Sugawara K, Harada M, Kusano T: Molecular cloning, characterization and expression of an elongation factor 1α gene in maize. Plant Mol Biol 29: 611–615 (1995).
Berchtold H, Reshetnikova L, Reiser COA, Schirmer NK, Sprinzl M, Hilgenfeld R: Crystal structure of active elongation factor Tu reveals major domain rearrangements. Nature 365: 126–132 (1993).
Bolle C, Sopory S, Lübberstedt Th, Herrmann RG, Oelmüller R: Segments emcoding 5′-untranslated leaders of genes for thylakoid proteins contain cis-elements essential for transcription. Plant J. 6: 513–523 (1994).
Bourne HR, Sanders DA, McCormick F: The GTPase superfamily: a conserved switch for diverse cell function. Nature 348: 125–132 (1990).
Bourne HR, Sanders DA, McCormick F: The GTPase superfamily: a conserved structure and molecular mechanism. Nature 349: 117–127 (1991).
Brands JH, Maassen JA, Hemert FJv, Amons R, Moller W: The primary structure of the alpha subunit of human elongation factor 1. Structural aspects of guanine-nucleotide-binding sites. Eur J Biochem 155: 167–171 (1986).
Browning KS: The plant translational apparatus. Plant Mol Biol 32: 107–144 (1996).
Creti R, Citarella F, Tiboni O, Sanangelantoni A, Palm P, Cammarano P: Nucleotide sequence of a DNA region comprising the gene for elongation factor 1α (EF-1α) from the ultrathermophilic archaeote Pyrococcus woesei: phylogenetic implications. J Mol Evol 33: 332–342 (1991).
Dié MK, Mazabraud A, Viel A, le Maire M, Denis H, Crawford E, Brown D: Three genes under different developmental control encode elongation factor 1-α in Xenopus laevis. Nucl Acids Res 18: 3489–3493 (1990).
Dunn MA, Morris A, Jack PL, Hughes MA: A lowtemperature-responsive translation elongation factor 1α from barley (Hordeum vulgare L.). Plant Mol Biol 23: 221–225 (1993).
Durso NA, Cyr RJ: Acalmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1α. Plant Cell 6: 893–905 (1994).
Ejiri S-i: Meth Enzymol 118: 140–153 (1986).
Ejiri S-i, Kawamura R, Katsumata T: Interactions among four subunits of elongation factor 1 from rice embryo. Biochim Biophys Acta 1217: 266–272 (1994).
Habben JE, Kirleis AW, Larkins BA: The origine of lysinecontaining proteins in opaque-2 maize endosperm. Plant Mol Biol 23: 825–838 (1993).
Joshi CP: Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucl Acids Res 15: 9627–9640 (1987).
Kamiie K, Taira H, Ooura H, Matsumoto S, Ejiri S, Katsumata T: Nucleotide sequencing of the cDNA encoding silk gland elongation factor 1α. Nucl Acids Res 21: 742 (1993).
Kjeldgaard M, Nyborg J: Refined sturcture of elongation factor EF-Tu from Escherichia coli. J Mol Biol 223: 721–742 (1992).
Kushiro M, Shimizu M, Tomita K: Molecular cloning and sequence determination of the tuf gene coding for the elongation factor Tu of Thermus thermophilus HB8. Eur J Biochem 170: 93–98 (1987).
Lechner K, Böck A: Cloning and nucleotide sequence of the gene for an archaebacterial protein synthesis elongation factor Tu. Mol Gen Genet 208: 523–528 (1987).
Matsumoto S, Oizumi N, Taira H, Ejiri S-i: Cloning and sequencing of the cDNA encoding rice elongation factor 1β′. FEBS Lett 311: 46–48 (1992).
Matsumoto S, Terui Y, Xi S, Taira H, Ejiri S-i: Cloning and characterization of the cDNA encoding rice elongation factor 1β. FEBS Lett 338: 103–106 (1994).
Morelli JK, Shewmaker CK, Vayda ME: Biphasic stimulation of translational activity correlates with induction of translation elongation factor 1 subunit a upon wounding in potato tubers. Plant Physiol 106: 897–903 (1994).
Nagata S, Nagashima K, Tsunetsugu-Yokota Y, Fujimura K, Miyazaki M, Kaziro Y: Polypeptide chain elongation factor 1α (EF-1α) from yeast: nucleotide sequence of one of the two genes for EF-1α from Saccharomyces cerevisiae. EMBO J 3: 1825–1830 (1984).
Ohta K, Toriyama M, Miyazaki M, Murofushi H, Hosoda S, Endo S, Sakai H: The mitotic apparatus-associated 51-kDa protein from sea urchin eggs is a GTP-binding protein and is immunologically related to yeast polypeptide elongation factor 1α. J Biol Chem 265: 3240–3247 (1990).
Oizumi N, Matsumoto S, Taira H, Ejiri S-i: Nucleotide sequences of the cDNA encoding wheat elongation factor 1β′. Nucl Acids Res 20: 5225 (1992).
Palmiter RD: Magnesium precipitation of ribonucleoprotein complexes: expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid. Biochemistry 13: 3606 (1974).
Rao TR, Slobin LI: The stability of mRNA for eukaryotic elongation factor Tu in friend erythro-leukemia cells varies with growth rate. Mol Cell Biol 8: 1085–1092 (1988).
Roth WW, Bragg PW, Corrias MV, Reddy NS, Dholakia JN, Wahba AJ: Expression of a gene for mouse eukaryotic elongation factor Tu during murine erythroleukemic cell differentiation. Mol Cell Biol 7: 3929–3936 (1987).
Saitou N, Nei M: The Neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 (1987).
Shepherd JCW, Walldorf U, Hug P, Gehring WG: Fruit flies with additional expression of the elongation factor EF-1α live longer. Proc Natl Acad Sci USA 74: 5463–5467 (1989).
Shewmaker CK, Ridge NP, Pokalsky AR, Rose RE, Hiatt WR: Nucleotide sequence of an EF-1α genomic clone from tomato. Nucl Acids Res 18: 4276 (1990).
Shiina N, Gotoh Y, Kubomura N, Iwamatsu A, Nishida E: Microtubule severing by elongation factor 1α. Science 266: 282–285 (1994).
Shure M, Wessler S, Fedoroff N: Molecular identification and isolation of the waxy locuc in maize. Cell 35: 225–233 (1983).
Thompson JD, Higgins DG, Gibson TJ: CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucl Acids Res 22: 4673–4680 (1994).
Toriyama K, Hinata K: Cell suspension and protoplast culture in rice. Plant Sci 41: 179–183 (1985).
Uchimiya H, Kidou S, Shimazaki T, Aotsuka S, Takamatsu S, Nishi R, Hashimoto H, Matsubayashi Y, Kidou N, Umeda M, Kato A: Random sequencing of cDNA libraries reveals a versatility of expressed genes in cultured cells of rice (Oryza sativa L.). Plant J 2: 1005–1009 (1992).
Ursin VM, Irvine JM, Hiatt WR, Shewmaker CK: Developmental analysis of elongation factor-1α expression in transgenic tobacco. Plant Cell 3: 583–591 (1991).
van Damme HTF, Amons R, Karssies R, Timmers CJ, Janssen GMC, Moller W: Elongation factor 1β of Artemia: localization of functional sites and homology to elongation factor 1δ. Biochim Biophys Acta 1050: 241–247 (1990).
Vayda ME, Shewmaker CK, Morelli JK: Translational arrest in hypoxic potato tubers is correlatedwith the aberrant association of elongation factor EF-1α with polysomes. Plant Mol Biol 28: 751–757 (1995).
Wang H, Parent M, Morais R: Cloning and characterizaion of a cDNA encoding elongation factor 1α from chicken cells devoid of mitochondrial DNA. Gene 140: 155–161 (1994).
Wu L, Ueda T, Messing J: 3'-end processing of the maize 27 kDa zein mRNA. Plant J 4: 535–544 (1993).
Xie Y, Wu R: Nucleotide sequence of a ribrose-1,5-bisphosphate carboxylase/oxygenase small subunit gene (rbcS) in rice. Nucl Acids Res 16: 7749 (1988).
Yang F, Demma N, Warren V, Dharmawardhane S, Condeelis J: Identification of an actin-binding protein fromDictyostelium as elongation factor 1α. Nature 347: 494–496 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kidou, Si., Ejiri, Si. Isolation, characterization and mRNA expression of four cDNAs encoding translation elongation factor 1A from rice (Oryza sativa L.). Plant Mol Biol 36, 137–148 (1998). https://doi.org/10.1023/A:1005960721762
Issue Date:
DOI: https://doi.org/10.1023/A:1005960721762