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Detection and identification of gibberellins in needles of silver fir (Abies alba Mill.) by combined gas chromatography-mass spectrometry

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Abstract

Extracts of mature silver fir (Abies alba Mill.) needles, current-year, and one-year old (A) and seven to nine-year old (B), were purified by reversed and normal phase HPLC. Gibberellin (GA)-like compounds were detected by the Tan-ginbozu dwarf rice micro-drop bioassay and corresponding fractions were analyzed by GC-MS. GA9 was present in small amounts, while a major component was a cellulase-hydrolysable GA9 conjugate which was assumed to be GA9 glucosyl ester. It is proposed that GA9 glucosyl ester plays a key role in the regulation of endogenous GA levels in silver fir needles.

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Authors and Affiliations

  1. Institut für Botanik, Universität Hohenheim (210), Garbenstraße 30, D-70593, Stuttgart, Germany

    Alexander Christmann

  2. INRA, Biochimie et Physiologie Moléculaire des Plantes, F-34060, Montpellier Cedex 1, France

    Patrick Doumas

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  1. Alexander Christmann
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Christmann, A., Doumas, P. Detection and identification of gibberellins in needles of silver fir (Abies alba Mill.) by combined gas chromatography-mass spectrometry. Plant Growth Regulation 24, 91–99 (1998). https://doi.org/10.1023/A:1005960610320

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  • DOI: https://doi.org/10.1023/A:1005960610320

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