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Isolation and characterization of two cyclin-like cDNAs from Arabidopsis

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Abstract

Cyclins are key regulators of a family of protein kinases called cyclin-dependent kinases (Cdks). Specific cyclins interact with specific Cdks to regulate the different transition points in the cell cycle. Six mitotic-like cyclins have previously been reported in Arabidopsis thaliana. Using polymerase chain reaction amplified cyclin-box sequences as probes, two new cyclin cDNAs are isolated from Arabidopsis. The deduced amino acid sequences of the isolated cDNAs (Arath;CycB1;3 and Arath;CycB1;4) show the highest sequence similarity with mitotic cyclins. Arath;CycB1;3 is most homologous to the plant CycB1group cyclins and contains a conserved motif that is typical of this group. Arath;CycB1;4, while homologous to Arath;CycB1;2, has some features that make it different from other known mitotic-like cyclins. These data suggest the presence of several distinct cyclins of CycB1 group in Arabidopsis. Analysis of expression of three members of CycB1 group (Arath;CycB1;2, Arath;CycB1;3 and Arath;CycB1;4) in different tissues by reverse transcription-polymerase chain reaction using primers corresponding to unique regions of their cDNAs shows that they are differentially expressed in different tissues.

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  1. Department of Biology and Program in Cell and Molecular Biology, Colorado State University, CO, 80523, USA

    Irene S. Day & A.S.N. Reddy

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  1. Irene S. Day
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  2. A.S.N. Reddy
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Day, I.S., Reddy, A. Isolation and characterization of two cyclin-like cDNAs from Arabidopsis. Plant Mol Biol 36, 451–461 (1998). https://doi.org/10.1023/A:1005959311462

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