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Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation

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Abstract

Expression of α-amylase genes during seedling development plays a key role in production of sugar from the starch stored in the cereal seed. Rice α-amylase Amy3D promoter/GUS constructs in transgenic rice cell lines were studied to identify cis elements in the promoter of this metabolite-regulated gene. Three sequences having the greatest effects on Amy3D gene expression included the amylase element (TATCCAT), the CGACG element, and a G box-related element (CTACGTGGCCA). These promoter cis elements are needed for high-level expression of Amy3D under conditions of sugar starvation. The involvement of G box cis-elements in environmental stress responses suggest a link between the nutrient stress and the environmental stress responses of the plant.

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Authors and Affiliations

  1. Section of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA

    Y.-S. Hwang, B.R. Thomas & R.L. Rodriguez

  2. Dept. of Immunology IMM-2, The Scripps Research Institute, La Jolla, CA, 92037, USA

    E.E. Karrer

  3. ICTAB/ORSTOM, Dept. of Cell Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA

    L. Chen

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  1. Y.-S. Hwang
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  4. L. Chen
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  5. R.L. Rodriguez
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Hwang, YS., Karrer, E., Thomas, B. et al. Three cis-elements required for rice α-amylase Amy3D expression during sugar starvation. Plant Mol Biol 36, 331–341 (1998). https://doi.org/10.1023/A:1005956104636

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