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Polyamine concentrations in four Poa species, differing in their maximum relative growth rate, grown with free access to nitrate and at limiting nitrate supply

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Abstract

Polyamines are thought to play a role in the control of inherent or environmentally-induced growth rates of plants. To test this contention, we grew plants of four grass species, the inherently fast-growing Poa annua L. and Poa trivialis L. and the inherently slow-growing Poa compressa L. and Poa pratensis (L.) Schreb., at three levels of nitrate supply. Firstly, plants were compared when grown with free access to nitrate, allowing the plants to grow at their maximum relative growth rate (RGRmax). Secondly, we compared the plants when grown with relative nitrate addition rates of 100 and 50 mmol N (mol N)−1 day−1 (RAR100 and RAR50, respectively).

The freely-occurring polyamines, spermine, spermidine and putrescine, were separated from their conjugates; the latter were further subdivided into a TCA-soluble and a TCA-insoluble fraction. Each of the three fractions responded differently to the nitrate supply. Under nitrogen limitation, the total concentration of polyamines (free and bound ones together) decreased in both leaves and roots of all Poa species, whereas that in the stem remained more or less the same. These effects were to a large extent determined by the free polyamines. For the conjugates there was more differentiation, both between plant organ and among polyamine structures. A positive correlation between the RGR, LAR (leaf area per plant mass), SLA (leaf area per leaf mass), LMR (leaf mass per plant mass) and SMR (stem mass per plant mass) with the polyamine concentration was found. The RMR (root mass per plant mass) showed a negative one. No significant differences were found between the inherently fast- and slow-growing grass species.

The (putrescine)/(spermine + spermidine) ratio in the leaves increased with decreasing nitrate supply, which is associated with a decrease in leaf expansion, accounting for a decrease in LAR and SLA. For the roots, this ratio tended to decrease with decreasing nitrate supply, whereas for the stems the results were somewhat more variable.

We found no evidence for a crucial role of polyamines in the determination of inherent variation of growth in spite of a positive correlation of especially the free polyamines with growth parameters.

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References

  1. Bagni N, Serafini-Fracassini D and Torrigiani P (1982) Polyamines and cellular growth processes in higher plants. In: P Wareing (ed) Plant Growth Substances I, pp 473–482. London, UK

  2. Bate NJ, Rood SR and Blake TJ (1988) Gibberellins and heterosis in poplar. Can J Bot 66: 1148–1152

    Google Scholar 

  3. Biasi R, Bagni N and Costa G (1988) Endogenous polyamines in apple and their relationship to fruit set and fruit growth. Physiol Plant 73: 201–205

    Google Scholar 

  4. Bharti MVR and Sawhney RN (1996) Involvement of polyamines in resistance of wheat to Puccinia recondita. Phytochemistry 43: 1009–1013

    Google Scholar 

  5. Bonneau L, Carré M, Dreumont C and Martin-Tanguy J (1994) Polyamine metabolism during seedling development in rice. Plant Growth Regul 15: 83–92

    Google Scholar 

  6. Chapin FS III (1980) The mineral nutrition of wild plants. Ann Rev Ecol Syst 11: 233–260

    Google Scholar 

  7. Cho S (1983) Enhancement by putrescine of gibberellin-induced elongation in hypocotyls of lettuce seedlings. Plant Cell Physiol 24: 305–308

    Google Scholar 

  8. Deletang J (1974) Presence de caffeoyl putrescine, de caffeoyl spermidine et de dicaffeoyl spermidinechez Nicotiana tabacum. Ann Tabac 11: 123–170

    Google Scholar 

  9. Dijkstra P, Ter Reegen H and Kuiper PJC (1990) Relation between relative growth rate, endogenous gibberellins, and the response to applied gibberellic acid for Plantago major. Physiol Plant 79: 629–634

    Google Scholar 

  10. Di Tomaso J, Shaff J and Kochian L (1989) Putrescine induced wounding and its effect on membrane integrity and ion transport processes in roots of intact corn seedlings. Plant Physiol 90: 988–995

    Google Scholar 

  11. Dumortier F, Flores H, Shekhawat N and Galston A (1983) Gradients of polyamines and their biosynthetic enzymes in coleoptiles and roots of corn. Plant Physiol 72: 915–918

    Google Scholar 

  12. Egea-Cortines M and Mizrahi Y (1991) Polyamines in cell division, fruit set and development, and seed germination. In: Slocum RD and Flores HE (eds) Biochemistry and Physiology of Polyamines in Plants, pp 143–158. Boca Raton, USA

  13. Evans PT and Malmberg RL (1989) Do polyamines have role in plant development? Annu Rev Plant Physiol Plant Mol Biol 40: 235–269

    Google Scholar 

  14. Felix H and Harr J (1987) Association of polyamines to different parts of various plant species. Physiol Plant 71: 245–250

    Google Scholar 

  15. Fisher RA and Yates (1963) Statistical Tables for Biological, Argicultural and Medical Research, 6th eds Oliver & Boyd, Edinburgh, UK

    Google Scholar 

  16. Flores HE (1991) Changes in polyamine metabolism in response to abiotic stress. In: Slocum RD and Flores HE (eds) Biochemistry and Physiology of Polyamines in Plants pp 213– 228. Boca Raton, USA

  17. Galston AW (1983) Polyamines as modulators of plant development. BioSci 33: 382–388

    Google Scholar 

  18. Galston AW and Flores HE (1991) Polyamines and plant morphogenesis. In: Slocum RD and Flores HE (eds) Biochemistry and Physiology of Polyamines, pp 175–184. Boca Raton, USA

  19. Garnier E and Laurent G (1994) Leaf anatomy, specific leaf mass and water content in congeneric annual and perennial grass species. New Phytol 128: 725–736

    Google Scholar 

  20. Goldberg R and Perdrizet E (1984) Ratio of free to bound polyamines during maturation in mung-bean hypocotyl cells. Planta 161: 531–535

    Google Scholar 

  21. Grime JP and Hunt R (1975) Relative growth rate: its range and adaptive significance in a local flora. J Ecol 57: 553–563

    Google Scholar 

  22. Hamasaki N and Galston A (1990) The polyamines in Xanthium strumarium and their response to photoperiod. Photochem Photobiol 52: 181–186

    Google Scholar 

  23. Ingestad T (1981) Nutrition and growth of birch seedlings. II. N, K, P, Ca and Mg nutrition. Physiol Plant 45: 149–157

    Google Scholar 

  24. Kakkar RK and Rai VK (1993) Plant polyamines in flowering and fruit ripening. Phytochemistry 33: 1281–1288

    Google Scholar 

  25. Kaur-Sawhney R, Dai Y and Galston A (1985) Effect of inhibitors of polyamine biosynthesis on gibberellin-induced internode growth in light grown dwarf peas. Plant Cell Physiol 27: 253–260

    Google Scholar 

  26. Kuiper D and Staal M (1987) The effect of exogenous applied plant growth regulators on the physiological plasticity in Plantago major ssp. pleiosperma: Responses of growth, shoot to root ratio, and respiration. Physiol Plant 69: 651–658

    Google Scholar 

  27. Kuiper D, Kuiper PJC, Lambers H, Schuit JT and Staal M (1989) Cytokinin contents in relation to mineral nutrition and benzyladenine addition in Plantago major ssp. pleiosperma. Physiol Plant 75: 511–517

    Google Scholar 

  28. Lambers H and Dijkstra P (1987) A physiological analysis of genotypic variation in relative growth rate: Can growth rate confer ecological advantage? In: Van Andel J, Bakker JP and Snaydon RW (eds) Disturbance in Grassland, pp 237–252. Dordrecht, The Netherlands

  29. Lambers H and Poorter H (1992) Inherent variation in growth rate between higher plants: A search for physiological causes and ecological consequences. Adv Ecol Res 23: 87–261

    Google Scholar 

  30. Lambers H, Nagel OW and Van Arendonk JJCM (1995) The control of biomass partitioning in plants from “favourable” and “stressful” environments: A role for gibberellins and cytokinins. Bul J Plant Physiol 21: 24–32

    Google Scholar 

  31. Martin-Tanguy J (1984) The occurence and possible function of hydroxy cinnamoyl acid amines in plants. Plant Growth Regul 3: 381–399

    Google Scholar 

  32. Matilla AJ (1996) Polyamines and seed germination. Seed Sci Res 6: 81–93

    Google Scholar 

  33. Metzger JD and Hassebrock AT (1990) Selection and characterization og a gibberellin-deficient mutant of Thlaspi arvense L. Plant Physiol 94: 1655–1662

    Google Scholar 

  34. Nagel O, Konings H and Lambers H (1994) Growth rate, plant development and water relations of the ABA-deficient tomato mutant sitiens. Physiol Plant 92: 102–108

    Google Scholar 

  35. Niemann GJ, Pureveen JBM, Eijkel GB, Poorter H and Boon JJ (1992) Differences in relative growth rate in 11 grasses correlate with differences in chemical composition as determined by pyrolysis mass spectrometry. Oecologia 89: 567–573

    Google Scholar 

  36. Niemann GJ, Eijkel GB, Konings H, Pureveen JBM and Boon JJ (1993) Chemical differences between wildtype and gibberellin mutants of tomato determined by pyrolysis-mass spectrometry. Plant Cell Environ 16: 1059–1069

    Google Scholar 

  37. Niemann GJ, Pureveen JBM, Eijkel GB, Poorter H and Boon JJ (1995) Differential chemical allocation in plant adaptation: a PyMS studie of 24 species differing in relative growth rate. Plant Soil 175: 275–289

    Google Scholar 

  38. Poorter H (1989) Interspecific differences in relative growth rate: On ecological causes and physiological consequences. In: Lambers H, Cambridge ML, Konings H and Pons TL (eds) Variation in Growth Rate and Productivity of Higher Plants, pp 45–68. The Hague, The Netherlands

  39. Poorter H (1989) Plant growth analysis: Towards a synthesis of the classical and functional approach. Physiol Plant 75: 237– 244

    Google Scholar 

  40. Poorter H and Remkes C (1990) Leaf area ratio and net assimilation rate of 24 wild species differing in relative growth rate. Oecologia 83: 553–559

    Google Scholar 

  41. Poorter H, Remkes C and Lambers H (1990) Carbon and nitrogen economy of 24 wild species differing in relative growth rate. Plant Physiol 94: 621–627

    Google Scholar 

  42. Poorter H and Bergkotte M (1992) Chemical composition of 24 wild species differing in relative growth rate. Plant Cell Environ 15: 221–229

    Google Scholar 

  43. Rey M, Diaz-Sala C and Rodriguez R (1994) Comparison of endogenous polyamine content in hazel leaves and buds between the annual dormancy and flowering phases of growth. Physiol Plant 91: 45–50

    Google Scholar 

  44. Rodriguez A, Canal M and Sanchez Tames R (1988) Indoleacetic acid, abscisic acid and phenolic substances during development of hazel leaves. Physiol Plant 73: 92–96

    Google Scholar 

  45. Rood SB, Pharis RP, Koshioka M and Major DJ (1983) Gibberellin and heterosis in maize. I. Endogenous gibberellin-like substances. Plant Physiol 71: 639–644

    Google Scholar 

  46. Rood SB and Pharis RP (1987) Hormones and heterosis in plants. In: Davies PJ (ed) Plant Hormones and Their Role in Plant Growth and Developement, pp 463–473 Dordrecht, The Netherlands

  47. Rood SB, Buzzell RI, Major DJ and Pharis RP (1990) Gibberellins and heterosis in maize, Quantitative relationships. Crop Sci 30: 281–286

    Google Scholar 

  48. Rood SB, Pearce DP, Williams PH and Pharis RP (1990) A. gibberellin-deficient Brassica mutant-rosette. Plant Physiol 89: 482–487

    Google Scholar 

  49. Rood SB, Zanewich KP and Bray DF (1990) Growth and development of Brassica genotypes differing in endogenous gibberellin content. II. Gibberellin content, growth analyses and cell size. Physiol Plant 79: 679–685

    Google Scholar 

  50. Ryser P and Lambers H (1995) Root and leaf attributes accounting for the performance of fast-and slow-growing grasses at different nutrient supply. Plant Soil 170: 251–265

    Google Scholar 

  51. Santerre A, Markiewicz M and Villanueva VR (1990) Effect of acid rain on polyamines in Picea. Phytochemistry 29: 1767– 1769

    Google Scholar 

  52. Serafini-Fracassini D and Mossetti U (1985) Free and bound polyamines in different physiological stages of Helianthus tuberosis tuber. In: L. Selmeci L, Brosnan M and Seiler N (eds) Recent progress in polyamine research, pp 551–560. Akademiai Kiado, Budapest and VNU, The Netherlands

  53. Serafini-Fracassini D and Mossetti U (1986) What is the function of conjugated polyamines in plants? In: Caldarera C, Clo C and Guarnieri C (eds) Biomedical studies of natural polyamines, pp 197–202. Bologna, France

  54. Slocum R, Kaur-Sawhney R and Galston AW (1984) The physiology and biochemistry of polyamines in plants. Arch Biochem Biophys 235: 283–303

    Google Scholar 

  55. Smith TA and Best GR (1977) Polyamines in barley seedlings. Phytochemistry 16: 841–843

    Google Scholar 

  56. Smith TA (1982) The function and metabolism of polyamines in higher plants. In: Wareing PF (ed) Plant Growth Substances, pp 463–472. London, UK

  57. Smith TA (1985) Polyamines. Annu Rev Plant Physiol 36: 117–143

    Google Scholar 

  58. Smith M, Davies P and Reid J (1985) Role of polyamines in gibberellin-induced internode growth in peas. Plant Physiol 78: 92–99

    Google Scholar 

  59. Smith M (1990) The involvement of polyamines in the genetic and gibberellin acid control of internodal growth in peas. In: Flores HE, Arteca RN and Shannon JC (eds) Polyamine and ethylene: Biochemistry, Physiology and Interactions, pp 101– 111. Rockville, USA

  60. Torrigiani P, Altamura M, Pasqua G, Monacelli B, Srefini-Fracassini D and Bagni N (1987) Free and conjugated polyamines during de novo floral and vegetative bud formation in thin cell layers of tobacco. Physiol Plant 70: 453–460

    Google Scholar 

  61. Van Arendonk JJCM and Poorter H (1994) The chemical composition and anatomical structure of leaves of grass species differing in relative growth rate. Plant Cell Environ 17: 963– 970

    Google Scholar 

  62. Van Arendonk JJCM, Niemann GJ, Boon JJ and Lambers H (1997) Effects of N-supply on anatomy and chemical composition of leaves of four grass species, belonging to the genus Poa, as determined by image-processing analysis and pyrolysismass spectrometry. Plant Cell Environ 20: 881–897

    Google Scholar 

  63. Van der Werf A, Schieving F and Lambers H (1993) Evidence for optimal partitioning of biomass and nitrogen at the range of nitrogen availabilities for fast-and slow-growing species. Func Ecol 7: 63–74

    Google Scholar 

  64. Wang S and Faust M (1993) Comparison of seasonal growth and polyamine content in shoots of orchard grown standard and genetic dwarf apple trees. Physiol Plant 89: 376–380

    Google Scholar 

  65. Watson MB and Malmberg RL (1996) Regulation ofArabidopsis thaliana (L.) Heynh arginine decarboxylase by potassium deficiency stress. Plant Physiol 111: 1077–1083

    Google Scholar 

  66. West C, Briggs GE and Kidd F (1920) Methods and significant relations in quantitative analysis of plant growth. New Phytol 19: 200–207

    Google Scholar 

  67. Willadino L, Camara T, Boget N, Claparols I, Santos M and Torné JM (1996) Polyamine and free amino acid variations in NaCl-treated embryogenic maize callus from sensitive and resistant culticars. J Plant Physiol 149: 179–185

    Google Scholar 

  68. Wyss-Benz M, Streit I and Ebert E (1988) Hydroxycinnamoyl amides in stem explants from flowering and non-flowering Nicotiana tabacum. Physiol Plant 74: 294–298

    Google Scholar 

  69. Zanewich KP, Rood SB and Williams PH (1990) Growth and development of Brassica genotypes differing in endogenous gibberellin content. II. Leaf and reproductive development. Physiol Plant 79: 673–678

    Google Scholar 

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Authors and Affiliations

  1. Department of Plant Ecology and Evolutionary Biology, Utrecht University, P.O. Box 800.84, 3508 TB, Utrecht, The Netherlands

    Jeroen J.C.M. Van Arendonk, Hans Lambers & Hans Lambers

  2. Bulgarian Academy of Sciences, Acad., Acad., 1113, Sofia, Bulgaria

    Emanuil Karanov & Vera Alexieva

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  1. Jeroen J.C.M. Van Arendonk
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  2. Emanuil Karanov
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  3. Vera Alexieva
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  4. Hans Lambers
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Van Arendonk, J.J., Karanov, E., Alexieva, V. et al. Polyamine concentrations in four Poa species, differing in their maximum relative growth rate, grown with free access to nitrate and at limiting nitrate supply. Plant Growth Regulation 24, 77–89 (1998). https://doi.org/10.1023/A:1005946330580

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