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Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum

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Abstract

Five cDNA clones encoding the catalytic subunits of Ser/Thr protein phosphatases (PP) of Nicotiana tabacum were described. Among them, three clones (NPP1, NPP2, and NPP3) encoded type 1 PP (PP1), whereas the rest of the clones (NPP4 and NPP5) encoded type 2A PP (PP2A). These cDNA clones exhibited high sequence identity in the PP core region to the corresponding genes from animals and plants. NPP1 mRNA was predominantly expressed in flowers, whereas NPP5 mRNA was mainly detected in leaves and flowers. In contrast, the transcripts of NPP2, NPP3, and NPP4 genes were present in all tissues examined. Throughout flower development, NPP1, NPP2, NPP5 mRNAs were expressed without any significant variation at the steady-state level. Genomic Southern blot showed that tobacco genome contained multiple genes and/or pseudogenes for both type 1 and type 2A phosphatases.

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Author notes

  1. Mi Chung Suh

    Present address: Department of Botany and Plant Pathology, Michigan State University, East Lansing, MI, 48824, USA

Authors and Affiliations

  1. Plant Cell and Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon, 305-600, Korea

    Hye Sun Cho, Youn Sung Kim, Jang Ryol Liu & Hyun-Sook Lee

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  1. Mi Chung Suh
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Chung Suh, M., Cho, H.S., Sung Kim, Y. et al. Multiple genes encoding serine/threonine protein phosphatases and their differential expression in Nicotiana tabacum. Plant Mol Biol 36, 315–322 (1998). https://doi.org/10.1023/A:1005943805988

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