Abstract
In order to identify genes involved in expression of desiccation tolerance in the foliage of the grass Sporobolus stapfianus, a cDNA library was constructed from desiccated leaf tissue of S. stapfianus. Differential screening resulted in the isolation of a number of clones which detect transcripts whose abundance alters during drought stress and the associated induction of desiccation tolerance. The characteristics of 6 of these cDNA clones are presented here. Transcripts represented by three of the cDNA clones accumulate during drying and following treatment of leaves with abscisic acid (ABA). A fourth cDNA clone detects a transcript which also accumulates following application of ABA but the transcript level fluctuates during drying. The remaining two cDNA clones are not responsive to ABA but transcript levels are present throughout the drying process. Characterisation of these cDNAs has led to the identification of the encoded proteins. Some have similarity to proteins which are known to be involved in the protection of cellular organelles and detoxification processes and they include dehydrin, LEA group 3 and thiol proteases which have been identified in other systems and shown to be induced by water stress. In addition one clone showed similarity to glyoxalase I, an enzyme involved in the removal of toxic byproducts produced during glycolysis, which has been shown to be induced by salt stress in tomato.
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References
Baker J, Steele C and Dure III L (1988) Sequence and characterisation of 6 LEA proteins and their genes from cotton. Plant Mol Biol 11: 277-291
Bartels D, Schneider K, Terstappen G, Piatkowski D and Salamini F (1990) Molecular cloning of abscisic acidmodulated genes which are induced during desiccation of the resurrection plant Craterostigma plantagineum. Planta 181: 27-34
Chen C and Clarke AE (1992) Proline rich protein from Nicotiana alata. International Patent WO92/20713
Close TJ (1996) Dehydrins: Emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plantar 97: 795-803
Close TJ, Kortt AA and Chandler PM (1989) A complementary DNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol 13: 95-108
Curry J, Morris CF and Walker-Simmons MK (1991) Sequence analysis of a cDNA encoding a group 3 LEA mRNA inducible by ABA or dehydration in wheat. Plant Mol Biol 16: 1073- 1076
Doring V, Schleicher M and Noegel AA (1991) Dictyostelium Annexin VII (Synexin); DNA sequence and isolation of a gene disruption mutant. The J Biol Chem 266: 17509-17515
Dure III L (1993) Structural motifs in LEA proteins of higher plants. In: Close TJ and Bray EA (eds) Responses of Plants to Cellular Dehydration during Environmental Stress, pp 91-103. Rockville, MD: Am Soc Plant Physiol
Espartero J, Sanchez-Aguayo I and Pardo JM(1995) Molecular characterisation of glyoxalase I from higher plants; upregulation by stress. Plant Mol Biol 29: 1223-1233
Gaff DF (1971) The desiccation tolerant higher plants of southern Africa. Science 174: 1033-1034
Gaff DF and Ellis RP (1974) Southern African grasses with foliage that revives after dehydration. Bothalia 11: 305-308
Gaff DF and Loveys BR (1984) Abscisic acid content and effects during dehydration of detached leaves of desiccation tolerant plants. J Exp Bot 35: 1350-1358
Gaff DF and Loveys BR (1993) Abscisic acid levels in drying plants of a resurrection grass. Trans Malaysian Soc Plant Physiol 3: 286-287
Gaff DF, Bartels D, Gaff JL and Schneider K (1993) Gene expression at low RWC in two hardy tropical grasses. Trans Malaysian Soc Plant Physiol 3: 238-240
Ghasempour HR, Anderson EM, Gianello RD and Gaff DF (1998) Growth inhibitor effects on protoplasmic drought tolerance and protein synthesis in the leaf cells of the resurrection grass Sporobolus stapfianus. Plant Growth Reg 24: 179-183
Ghasempour HR, Gaff DF, Williams RP and Gianello RD (1998) Contents of sugars in leaves of drying desiccation tolerant flowering plants, particularly grasses. Plant Growth Reg 24: 185-192
Gerke V (1991) Identification of a homologue for Annexin VII (Synexin) in Dictyostelium discoideum. The J Biol Chem 266: 1697-1700
Greenwood M and Tsang A (1991) Sequence and expression of annexin VII of Dictyostelium discoideum. Biochim Biophys Acta 1088: 429-432
Kuang J, Gaff DF, Gianello RD, Blomstedt CK, Neale AD and Hamill JD (1995) Changes in in vivo protein complements in drying leaves of the desiccation-tolerant grass Sporobolus stapfianus and the desiccation-sensitive grass Sporobolus pyramidalis. Aust J Plant Physiol 22: 1027-34
Lan Y, Lu T, Lovett PS and Creighton DJ (1995) Evidence for a (Triosephosphate Isomerase-like) "catalytic loop" near the active site of Glyoxalase I. The J Biol Chem 270: 12957- 12960
Noegel AA, Gensch G, Lottspeich F and Schleicher M (1990) A protein with homology to the C-terminal repeat sequence of octopus rhodopsin and synaptophysin is a member of a multigene family in Dictyostelium discoideum. FEBS Letters 266: 118-122
Ovchinnikov YA, Abdulaev NG, Zolotarev AS, Artamonov D, Bespalov IA, Dergachev AE and Tsuda M (1988) Octopus rhodopsin: Amino acid sequence deduced from cDNA. FEBS Letters 232: 69-72
Piatkowski D, Schneider K, Salamini F and Bartels D (1990) Characterisation of five abscisic acid-responsive cDNA clones isolated fromthe desiccation tolerant plant Craterostigma plantagineum and their relationship to other water-stress genes. Plant Physiol 94: 1682-1688
Sambrook J, Fritsch EF and Maniatis T (1989) Molecular cloning, a laboratory manual. New York, USA: Cold Spring Harbor Lab. Press
Schneider K, Wells B, Schmelzer E, Salamini F and Bartels D (1993) Desiccation leads to the rapid accumulation of both cytosolic and chloroplastic proteins in the resurrection plant Craterostigma plantagineum Hochst. Planta 189: 120-131
Verwoerd TC, Dekker BMM and Hoekema A (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acid Research 17: 2362
Whittier RF, Dean DA and Rogers JC (1987) Nucleotide sequence analysis of alpha-amylase and thiol protease genes that are normally regulated in barley aleurone cells. Nucleic Acid Research 15: 2515-2535
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Blomstedt, C., Gianello, R., Hamill, J. et al. Drought-stimulated genes correlated with desiccation tolerance of the resurrection grass Sporobolus stapfianus. Plant Growth Regulation 24, 153–161 (1998). https://doi.org/10.1023/A:1005923528109
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DOI: https://doi.org/10.1023/A:1005923528109