Advertisement

Photosynthesis Research

, Volume 55, Issue 2–3, pp 349–355 | Cite as

Comparative analysis of the primary structure of the reaction center-bound cytochrome subunit in purple bacteria

  • Kenji V. P. Nagashima
  • Yumiko Sakuragi
  • Keizo Shimada
  • Katsumi Matsuura
Article

Abstract

The amino acid sequences of the reaction center-bound cytochrome subunit of six species of purple bacteria were compared. Amino acid residues thought to be important in controlling the redox midpoint potentials of four hemes in Blastochloris (Rhodopseudomonas) viridis were found to be well conserved. As opposed to all other species studied, the amino acid sequence of the cytochrome subunit of B. viridis had several insertions of more than 10 residues at specific regions close to the LM core, suggesting that interaction of the cytochrome subunit with the LM core in most species is different from that in B. viridis. Distribution of charged amino acid residues on the surface of the cytochrome subunit was compared among six species and discussed from the viewpoint of interaction with soluble electron donors.

amino acid sequence charge interaction cyclic electron transfer cytochrome purple photosynthetic bacteria reaction center 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Deisenhofer J, Epp O, Miki K, Huber R and Michel H (1985) Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3 Å resolution. Nature 318: 618–624Google Scholar
  2. Deisenhofer J, Epp O, Sinning I and Michel H (1995) Crystallographic refinement at 2.3Å resolution and refined model of the photosynthetic reaction centre from Rhodopseudomonas viridis. J Mol Biol 246: 429–457Google Scholar
  3. Dracheva SM, Drachev LA, Konstantinov AA, Semenov AY, Skulachev VP, Arutjunjan AM, Shuvalov VA and Zaberezhnaya SM (1988) Electrogenic steps in the redox reactions catalyzed by photosynthetic reaction-centre complex from Rhodopseudomonas viridis. Eur J Biochem 171: 253–264Google Scholar
  4. Fukushima A, Matsuura K, Shimada K and Satoh T (1988) Reaction center-B880 pigment protein complexes with bound cytochrome c-555 and c-551 from Rhodocyclus gelatinosus. Biochim Biophys Acta 933: 399–405Google Scholar
  5. Gunner MR and Honig B (1991) Electrostatic control of midpoint potentials in the cytochrome subunit of the Rhodopseudomonas viridis reaction center. Proc Natl Acad Sci USA 88: 9151–9155Google Scholar
  6. Hochkoeppler A, Ciurli S, Venturoli G and Zannoni D (1995) The high potential iron–sulfur protein (HiPIP) from Rhodoferax fermentans is competent in photosynthetic electron transfer. FEBS Lett 357: 70–74Google Scholar
  7. Knaff DB, Willie A, Long JE, Kriauciunas A, Durham B and Millett F (1991) Reaction of cytochrome c 2 with photosynthetic reaction centers from Rhodopseudomonas viridis. Biochemistry 30: 1303–1310Google Scholar
  8. Matsuura K, Fukushima A, Shimada K and Satoh T (1988) Direct and indirect electron transfer from cytochromes c and c 2 to the photosynthetic reaction center in pigment–protein complexes isolated from Rhodocyclus gelatinosus. FEBS Lett 237: 21–25Google Scholar
  9. Nagashima KVP, Itoh S, Shimada K and Matsuura K (1993) Photooxidation of reaction center-bound cytochrome c and generation ofmembrane potential determined by carotenoid band shift in the purple photosynthetic bacterium, Rhodospirillum molischianum. Biochim Biophys Acta 1140: 297–303Google Scholar
  10. Nagashima KVP, Matsuura K, Ohyama S and Shimada K (1994) Primary structure and transcription of genes encoding B870 and photosynthetic reaction center apoproteins from Rubrivivax gelatinosus. J Biol Chem 269: 2477–2484Google Scholar
  11. Nagashima KVP, Shimada K and Matsuura K (1996a) Shortcut of the photosynthetic electron transfer in a mutant lacking of the reaction center-bound cytochrome subunit by gene disruption in purple bacterium, Rubrivivax gelatinosus. FEBS Lett 385: 209–213Google Scholar
  12. Nagashima KVP, Matsuura K and Shimada K (1996b) The nucleotide sequence of the puf operon from the purple photosynthetic bacterium, Rhodospirillum molischianum: Comparative analyses of light-harvesting proteins and the cytochrome subunits associated with the reaction centers. Photosynth Res 50: 61–70Google Scholar
  13. Nagashima KVP, Hiraishi A, Shimada K and Matsuura K (1997) Horizontal transfer of genes coding for the photosynthetic reaction centers of purple bacteria. J Mol Evol: 45: 131–136Google Scholar
  14. Nitschke W and Dracheva SM (1995) Reaction center associated cytochromes. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 775–805. Kluwer Academic Publisher, Dordrecht, the NetherlandsGoogle Scholar
  15. Osyczka A, Yoshida M, Nagashima KVP, Shimada K and Matsuura K (1997) Electron transfer from high-potential iron–sulfur protein and low-potential cytochrome c-551 to the primary donor of Rubrivivax gelatinosus reaction center mutationally devoided of the bound cytochrome subunit. Biochim Biophys Acta 1321: 93–99Google Scholar
  16. Pugsley AP (1993) The complete general secretory pathway in gram-negative bacteria. Microbiol Rev 57: 50–108Google Scholar
  17. Schoepp B, Parot P, Menin L, Gaillard J, Richaud P and Vermeglio A (1995) In vivo participation of a high potential iron–sulfur protein as electron donor to the photochemical reaction center of Rubrivivax gelatinosus. Biochemistry 34: 11736–11742Google Scholar
  18. Tiede DM, Vashishta A-C and Gunner MR (1993) Electrontransfer kinetics and electrostatic properties of the Rhodobacter sphaeroides reaction center and soluble c-cytochromes. Biochemistry 32: 4515–4531Google Scholar
  19. Vermeglio A, Richaud P and Breton J (1989) Orientation and assignment of the four cytochrome hemes in Rhodopseudomonas viridis reaction center. FEBS Lett 243: 259–263Google Scholar
  20. Wakao N, Shiba T, Hiraishi A, Ito M and Sakurai Y (1993) Distribution of bacteriochlorophyll a in species of the genus Acidiphilium. Curr Microbiol 27: 277–279Google Scholar
  21. Weyer KA, Lottspeich F, Gruenberg H, Lang F, Oesterhelt D and Michel H (1987a) Amino acid sequence of the cytochrome subunit of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis. EMBO J 6: 2197–2202Google Scholar
  22. Weyer KA, Schafer W, Lottspeich F and Michel H (1987b) The cytochrome subunit of the photosynthetic reaction center from Rhodopseudomonas viridis is a lipoprotein. Biochemistry 26: 2909–2914Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Kenji V. P. Nagashima
    • 1
  • Yumiko Sakuragi
    • 1
  • Keizo Shimada
    • 1
  • Katsumi Matsuura
    • 1
  1. 1.Department of BiologyTokyo Metropolitan UniversityTokyoJapan

Personalised recommendations