Abstract
An Arabidopsis cDNA clone encoding a DNA-binding protein, RAP-1, was isolated by southwestern screening of an Escherichia coli cDNA expression library. The protein contains a bHLH DNA-binding domain and is homologous to R proteins, regulating anthocyanin biosynthesis. RAP-1 binds to the sequence CACNTG. It is encoded by a single gene, which is expressed to high levels in root and stem and to low levels in leaf and flower. No expression could be detected in siliques. Rap-1 does not correspond to one of the known loci involved in anthocyanin biosynthesis, since it is located at a different map position. In contrast to the maize R protein Lc, RAP-1 did not induce anthocyanin biosynthesis in pea cotyledons. Thus, RAP-1 is a novel member of the bHLH class of DNA-binding proteins.
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References
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 215: 403-10 (1990).
Blackwell TK, Weintraub H: Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection. Science 250: 1104- 1110 (1990).
Ellenberger T, Fass D, Arnaud M, Harrison SC: Crystal structure of transcription factor E47: E-box recognition by a basic region helix-loop-helix dimer. Genes Devel 8: 970-980 (1994).
Ferré-D'Amaré AR, Prendergast GC, Ziff EB, Burley SK: Recognition by Max of its cognate DNA through a dimeric b/HLH/Z domain. Nature 363: 38-45 (1993).
Genetics Computer Group: Program Manual for theWisconsin Package, version 8 (1994).
Goff SA, Cone KC, Chandler VL: Functional analysis of the transcriptional activator encoded by the maize B gene: evidence for a direct functional interaction between two classes of regulatory proteins. Genes Devel 6: 864-875 (1992).
Kawagoe Y, Murai N: A novel basic region/helix-loop-helix protein binds to a G-box motif CACGTG of the bean seed storage protein β-phaseolingene. Plant Sci 116: 47-57 (1996).
Koes RE, Quattrocchio F, Mol JNM: The flavonoid biosynthetic pathway in plant: function and evolution. BioEssays 16: 123-132 (1994).
Lister C, Dean C: Recombinant inbred lines formapping RFLP and phenotypic markers in Arabidopsis thaliana. Plant J 4: 745-750 (1993).
Lloyd AM, Walbot V, Davis RW: Arabidopsisand Nicotianaanthocyanin production activated by maize regulators RandC1. Science 238: 1773-1775 (1992).
Ludwig SR, Bowen B, Beach L, Wessler SR: Aregulatory gene as a novel visible marker for maize transformation. Science 247: 449-450 (1990).
Ludwig SR, Wessler SR: Maize R gene family: tissue-specific helix-loop-helix proteins. Cell 62: 849-851 (1990).
Memelink J, Swords KMM, Staehelin LA, Hoge JHC: Southern, Northern and Western blot analysis. In: Gelvin SB, Schilperoort RA (eds) Plant Molecular Biology Manual, F1: 1-23.Kluwer Academic Publishers, Dordrecht (1994).
Meshi T, Iwabuchi M: Plant transcription factors. Plant Cell Physiol 36: 1405-1420 (1995).
de Pater S, Katagiri F, Kijne J, Chua N-H: bZIP proteins bind to a palindromic sequence without an ACGT core located in a seed-specific element of the pea lectin promoter. Plant J 6: 133-140 (1994).
de Pater S, Pham K, Chua N-H, Memelink J, Kijne J: A 22-bp fragment of the pea lectin promoter containing essential TGAC-like motifs confers seed-specific gene expression. Plant Cell 5: 877-886 (1993).
de Pater S, Pham K, Memelink J, Kijne J: Binding specificity and tissue-specific expression pattern of the ArabidopsisBzip transcription factor TGA2. Mol Gen Genet 250: 237-239 (1996).
Purugganan MD, Wessler SR: Molecular evolution of the plant Rregulatory gene family. Genetics 138: 849-854 (1994).
Shieh MW, Wessler SR, Raikhel NV: Nuclear targeting of the maize R protein requires two nuclear localization sequences. Plant Physiol 101: 353-361 (1993).
Shirley BW, Kubasek WL, Storz G, Bruggemann E, Koornneef M, Ausubel FM, Goodman HM: Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis. Plant J 8: 659-671 (1995).
Tuerck JA, Fromm ME: Elements of the maize A1promoter required for transactivation by the anthocyanin B/C1or phlobaphene Pregulatory genes. Plant Cell 6: 1655-1663 (1994).
Urao T, Yamaguchi-Shinozaki K, Mitsukawa N, Shibata D, Shinozaki K: Molecular cloning and characterization of a gene that encodes a MYC-related protein in Arabidopsis. PlantMol Biol 32: 571-576 (1996).
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de Pater, S., Pham, K., Memelink, J. et al. RAP-1 is an Arabidopsis MYC-like R protein homologue, that binds to G-box sequence motifs. Plant Mol Biol 34, 169–174 (1997). https://doi.org/10.1023/A:1005898823105
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DOI: https://doi.org/10.1023/A:1005898823105