Abstract
Young in vitro cultured panicles of maize(Zea mays L.) isolated from plants previously infected under controlled conditions by Sporisorium reilianum (Kühn) Landon and Fullerton, the agent of head smut, were maintained in culture up to the regeneration of healthy plantlets. Production of plants remained low and depended on a synchronization of the date of inoculation with the physiological condition of the mother-plant, which is linked to the season. Use of fungicide throughout the experiment prevented the colonization by the parasite. Such a regeneration takes advantage of a vegetative reversal naturally induced by S. reilianum on certain spikelets. Our method produced regenerants whose characters are different from those of mother-plant: the panicle was always replaced by a fertile ear.
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Touraud, G., Bill, L. & Piollat, M. Phyllodied panicles caused by head smut of maize and vegetative multiplication of maize. Plant Cell, Tissue and Organ Culture 50, 19–26 (1997). https://doi.org/10.1023/A:1005887012907
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DOI: https://doi.org/10.1023/A:1005887012907