Plant Molecular Biology

, Volume 35, Issue 5, pp 655–660

Structural analysis of rDNA in the genus Nicotiana

  • Nikolai V. Borisjuk
  • Yurij M. Davidjuk
  • Stepan S. Kostishin
  • Galina P. Miroshnichenco
  • Riccardo Velasco
  • Vera Hemleben
  • Roman A. Volkov

Abstract

The nucleotide sequence of the intergenic spacer (IGS) region between the 25S and the 18S rRNA coding regions has been determined for tobacco (Nicotiana tabacum). The IGS (5140 bp in length) can be subdivided into several regions (I–VII) two of which, upstream and downstream of the putative transcription initiation site (TIS), contain prominent subrepeats (A and C). The unique sequence in the central part of the IGS (region IV) preceding the TIS is extremely AT-rich. The distance from the putative TIS to the 5′ end of the 18S rRNA gene is 3005 bp. The IGS sequences are compared with potato (Solanum tuberosum) and tomato (Lycopersicon esculentum) IGS. Restriction mapping of 13 Nicotiana species shows that considerable rDNA repeat length heterogeneity in this genus is probably due to different numbers of A and C subrepeats.

ribosomal DNA intergenic spacer tobacco molecular evolution Lycopersicon/ Solanum/ Solanaceae. 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Nikolai V. Borisjuk
    • 1
  • Yurij M. Davidjuk
    • 2
  • Stepan S. Kostishin
    • 2
  • Galina P. Miroshnichenco
    • 3
  • Riccardo Velasco
    • 4
  • Vera Hemleben
    • 4
  • Roman A. Volkov
    • 2
  1. 1.Institut für Pflanzengenetik und KulturpflanzenforschungGaterslebenGermany
  2. 2.Department of BiochemistryUniversity of ChernivtsyChernivtsyUkraine
  3. 3.Institute of Physical and Chemical BiologyUniversity of MoscowMoscowRussia
  4. 4.Lehrstuhl für Allgemeine Genetik, Biologisches InstitutUniversität TübingenTübingenGermany

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