Plant Molecular Biology

, Volume 35, Issue 5, pp 655–660 | Cite as

Structural analysis of rDNA in the genus Nicotiana

  • Nikolai V. Borisjuk
  • Yurij M. Davidjuk
  • Stepan S. Kostishin
  • Galina P. Miroshnichenco
  • Riccardo Velasco
  • Vera Hemleben
  • Roman A. Volkov


The nucleotide sequence of the intergenic spacer (IGS) region between the 25S and the 18S rRNA coding regions has been determined for tobacco (Nicotiana tabacum). The IGS (5140 bp in length) can be subdivided into several regions (I–VII) two of which, upstream and downstream of the putative transcription initiation site (TIS), contain prominent subrepeats (A and C). The unique sequence in the central part of the IGS (region IV) preceding the TIS is extremely AT-rich. The distance from the putative TIS to the 5′ end of the 18S rRNA gene is 3005 bp. The IGS sequences are compared with potato (Solanum tuberosum) and tomato (Lycopersicon esculentum) IGS. Restriction mapping of 13 Nicotiana species shows that considerable rDNA repeat length heterogeneity in this genus is probably due to different numbers of A and C subrepeats.

ribosomal DNA intergenic spacer tobacco molecular evolution Lycopersicon/ Solanum/ Solanaceae. 


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  1. 1.
    Borisjuk N, Hemleben V: Nuleotide sequence of potato rDNA intergenic spacer. Plant Mol Biol 21: 381–384 (1992).Google Scholar
  2. 2.
    Borisjuk N, Borisjuk L, Petjuch G, Hemleben V: Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae. Genome 37: 271–279 (1994).Google Scholar
  3. 3.
    Borisjuk NV, Kostishin SS, Volkov RA, Miroshnichenko GP: Ribosomal RNAgene organization in higher plants from genus Nicotiana. Mol Biol (USSR) 23: 1067–1074 (1989).Google Scholar
  4. 4.
    D'Arcy WG: The Solanaceae since 1976, with a review of its biogeography. In: Hawkes JG, Lester RN, Nee M, Estrada N (eds) Solanaceae III: Taxonomy, Chemistry, Evolution, pp. 75–138. Royal Botanic Garden Kew and Linnean Society, London (1991).Google Scholar
  5. 5.
    Doelling JH, Gaudino RJ, Pikaard CS: Functional analysis of Arabidopsis thaliana rDNA gene and spacer promoters in vivo and by transient expression. Proc Natl Acad Sci USA 90: 7528–7532 (1993).Google Scholar
  6. 6.
    Gerstner J, Schiebel K, Waldburg G, Hemleben V: Complex organization of the length heterogeneous 5′ external spacer of mung bean (Vigna radiata) ribosomal DNA. Genome 30: 723–733 (1988).Google Scholar
  7. 7.
    Goodspeed T, Thompson M: Cytotaxonomy of Nicotiana. Bot Rev 25: 385–415 (1959).Google Scholar
  8. 8.
    Gründler P, Unfried I, Pascher K, Schweizer D: rDNA intergenic region from Arabidopsis thaliana: structural analysis, intraspecific variation and functional implications. J Mol Biol 221: 1209–1222 (1991).Google Scholar
  9. 9.
    Hemleben V, Ganal M, Gerstner J, Schiebel K, Torres RA: Organization and length heterogeneity of plant ribosomal RNA genes. In: Kahl G (ed) The Architecture of Eukaryotic Gene, pp. 371–384. VHC, Weinheim (1988).Google Scholar
  10. 10.
    Hemleben V, Zentgraf U: Structural organization and regulation of transcription by RNA polymerase I of plant nuclear ribosomal RNA genes. In: Nover L (ed) Results and Problems in. Cell Differentiation 20: Plant Promoters and Transcription Factors, pp. 3–24. Springer-Verlag, Berlin/Heidelberg (1994).Google Scholar
  11. 11.
    Kelly RJ, Siegel A: The Cucurbita maxima ribosomal DNA intergenic spacer has a complex structure. Gene 80: 239–248 (1989).Google Scholar
  12. 12.
    King K, Torres RA, Zentgraf U, Hemleben V: Molecular evolution of the intergenic spacer in the nuclear ribosomal RNA genes of Cucurbitaceae. J Mol Evol 36: 144–152 (1993).Google Scholar
  13. 13.
    Narayan RKJ: Evolutionary significance of DNA variation in plants. Evol Trends Plants 2: 121–130 (1988).Google Scholar
  14. 14.
    Perry KL, Palukaitis P: Transcription of tomato ribosomal DNA and the organization of the intergenic spacer. Mol Gen Genet 221: 102–112 (1990).Google Scholar
  15. 15.
    Rogers SO, Bendich AJ: Ribosomal DNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol Biol 9: 509–520 (1987).Google Scholar
  16. 16.
    Sambrook J, Fritsch E, Maniatis T. Molecular Cloning. A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).Google Scholar
  17. 17.
    Volkov RA, Borisyuk NV, Kostishin SS, Miroshnichenko GP: rRNA genes rearrangement and speciation in Solanaceae. Comm Higher School Biol Sci (USSR) 10: 92–100 (1989).Google Scholar
  18. 18.
    Volkov RA, Borisjuk NV, Kostishin SS, Panchuk II: Variability of rRNA genes in Nicotiana correlates with the chromosome reconstruction. Mol Biol (Russia) 25: 442–450 (1991).Google Scholar
  19. 19.
    Volkov RA, Kostishin SS, Ehrendorfer F, Schweizer D: Organization and molecular evolution of rDNA external transcribed spacer region in two diploid relatives of Nicotiana tabacum (Solanaceae). Plant Syst Evol 201: 117–129 (1996).Google Scholar

Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Nikolai V. Borisjuk
    • 1
  • Yurij M. Davidjuk
    • 2
  • Stepan S. Kostishin
    • 2
  • Galina P. Miroshnichenco
    • 3
  • Riccardo Velasco
    • 4
  • Vera Hemleben
    • 4
  • Roman A. Volkov
    • 2
  1. 1.Institut für Pflanzengenetik und KulturpflanzenforschungGaterslebenGermany
  2. 2.Department of BiochemistryUniversity of ChernivtsyChernivtsyUkraine
  3. 3.Institute of Physical and Chemical BiologyUniversity of MoscowMoscowRussia
  4. 4.Lehrstuhl für Allgemeine Genetik, Biologisches InstitutUniversität TübingenTübingenGermany

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