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Erythromycin as a tool to investigate the tetrapyrrole biosynthetic pathways in habituated and normal sugarbeet calli

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Abstract

Erythromycin (ERT) has been shown to reduce the 5-aminolevulinic acid (ALA) synthesizing capacity of a normal (N) chlorophyllous sugarbeet callus, grown under light, in contrast to a habituated achlorophyllous non-organogenic (HNO) callus of the same species. Similar effects were obtained on total hemes and on catalase which is a hemoprotein used as marker. The effect of ERT, which is an inhibitor of plastid differentiation and of chlorophyll synthesis, was reversed in the N callus by a supply of glycine and succinate. The compounds are the precursors of ALA synthesized through 5-aminolevulinic acid synthase (ALAS) which is implied in the Shemin pathway. The involvement of ALAS appeared to be favoured when plastids were undifferentiated (HNO callus) or when plastids were inefficient (N callus under darkness or under light after ERT treatment).

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Authors and Affiliations

  1. Hormonologie Fondamentale et Appliquée, Institut de Botanique B 22, University of Liège, Sart Tilman, B – 4000, Liège, Belgium

    B. Bisbis, C. Kevers & Th. Gaspar

  2. Physiologie et Biochimie Végétales, University of Caen, IRBA, Esplanade de la Paix, F – 14032, Caen, France (*author for correspondence)

    C. Huault & J.P. Billard

Authors
  1. B. Bisbis
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  2. C. Kevers
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  3. C. Huault
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  4. J.P. Billard
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  5. Th. Gaspar
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Bisbis, B., Kevers, C., Huault, C. et al. Erythromycin as a tool to investigate the tetrapyrrole biosynthetic pathways in habituated and normal sugarbeet calli. Plant Growth Regulation 23, 147–152 (1997). https://doi.org/10.1023/A:1005854112134

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  • DOI: https://doi.org/10.1023/A:1005854112134

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