Abstract
Fluorescence detected magnetic resonance (FDMR) was used to study the lowest triplet state of bacteriochlorophylls (BChls) c and d in Chlorobium (Chl.) tepidum and Chl. vibrioforme, respectively. These pigments were studied both in the oligomeric form (in whole cells) and in the monomeric form (after conversion using a 1% 1-hexanol treatment). Fluorescence spectra show the presence of lower-state aggregates, apart from monomers, in samples treated with 1-hexanol. Values of the zero field splitting (ZFS) parameter D, obtained from FDMR spectra, were found to decrease with an increasing aggregate size. The observed ZFS trends are explained by a delocalization of the triplet spins, including a charge resonance (CR) contribution, over the aggregate. A simple model is presented relating the changes of D and E as a result of monomer aggregation to the aggregate geometry. Application of this model to BChls c and d indicates approximately diagonal stacking of the monomers in the dimer. Results for oligomeric BChl c and d were compared with those previously obtained for oligomeric BChl e. FDMR transitions of BChls c, d and e differ both in frequencies and in signs. The D and E values of Car's and BChl a (in whole cells) agree well with those reported for Chl. phaeobacteroides and Chl. limicola.
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Psencik, J., Schaafsma, T.J., Searle, G.F.W. et al. Fluorescence detected magnetic resonance of monomers and aggregates of bacteriochlorophylls of green sulfur bacteria Chlorobium sp.. Photosynthesis Research 52, 83–92 (1997). https://doi.org/10.1023/A:1005842231286
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DOI: https://doi.org/10.1023/A:1005842231286