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Expression of a receptor protein tyrosine phosphatase in human glial tumors

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Abstract

We have analyzed expression of a receptor protein tyrosine phosphatase (RPTPζ/β) in tissue samples from 23 human gliomas. Using the reverse transcription-polymerase chain reaction (RT-PCR) technique, we assayed for the presence or absence of mRNA transcripts encoding the intact receptor and 2 alternatively spliced forms of RPTPζ/β. Transcripts encoding the intact and truncated receptors were expressed in all of the lower grade gliomas (WHO grade 1–3) analyzed, but not in 55% of the grade 4 glioblastomas multiforme (GBM). However, this subset of GBMs did express an alternatively spliced secreted form comprised of only the RPTPζ/β extracellular domain. Our data suggests there may be a correlation between the loss of transcripts encoding the receptor forms of RPTPζ/β and progression from low to high grade gliomas. This work provides additional evidence for the importance of phosphatase isoform expression in human tumors.

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Authors and Affiliations

  1. Barrow Neurological Institute of St. Joseph's Hospital and Medical Center, Phoenix

    Sylvia A. Norman & John G. Golfinos

  2. Neuro-Oncology Research, Barrow Neurological Institute of St. Joseph's Hospital and Medical Center, Phoenix

    Adrienne C. Scheck

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  1. Sylvia A. Norman
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  2. John G. Golfinos
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  3. Adrienne C. Scheck
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Norman, S.A., Golfinos, J.G. & Scheck, A.C. Expression of a receptor protein tyrosine phosphatase in human glial tumors. J Neurooncol 36, 209–217 (1998). https://doi.org/10.1023/A:1005840420136

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