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Plant Cell, Tissue and Organ Culture

, Volume 48, Issue 3, pp 189–193 | Cite as

Somatic embryogenesis and plant regeneration in Anthurium scherzerianum

  • Musa Hamidah
  • Abdul Ghani Abdul Karim
  • Pierre Debergh
Article

Abstract

Several types of explants, growth regulators, sugars and gelling agents were tested to induce somatic embryogenesis in Anthurium scherzerianum Schott. For induction, leaf pieces from micropropagated plants were found to be the best explant on a medium with 18 μM 2,4-dichlorophenoxyacetic acid and a high sucrose concentration (6), solidified with Gelrite. Somatic embryos converted to entire plants on a medium with 0.46 μM kinetin. Plantlets were transferred to greenhouse conditions for evaluation of plant uniformity.

callus explant in vitro 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Musa Hamidah
    • 1
  • Abdul Ghani Abdul Karim
    • 2
  • Pierre Debergh
    • 1
  1. 1.Dept. Plant Production - HorticultureUniversity GentGentBelgium
  2. 2.Dept. Botany, Faculty of life SciencesNational University of MalaysiaBangi, SelangorMalaysia

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