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Expression in anthers of two genes encoding Brassica oleracea transmembrane channel proteins

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Abstract

Screening of an anther cDNA expression library resulted in the isolation of two almost identical cDNA clones, termed mipA and mipB, showing homology with sequences encoding transmembrane channel proteins from the MIP family. Both clones were expressed in several tissues, but not in pollen. MipA was preferentially expressed in the surrounding sporophytic tissues of stamens. Anthers subjected to drought were induced to accumulate even more mip transcripts, which was entirely due to higher mipA gene expression. On basis of isolation procedures, sequence homology and drought inducibility of mipA we conclude that the encoded proteins probably are constituents of the pollen coat and are aquaporins.

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Author notes

  1. René K. Ruiter

    Present address: Department of Brassica and Oilseeds Research, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK

Authors and Affiliations

  1. Department of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, Netherlands

    René K. Ruiter, Gerben J. van Eldik, Marinus M.A. van Herpen, Jan A.M. Schrauwen & George J. Wullems

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  1. René K. Ruiter
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  4. Jan A.M. Schrauwen
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Ruiter, R.K., van Eldik, G.J., van Herpen, M.M. et al. Expression in anthers of two genes encoding Brassica oleracea transmembrane channel proteins. Plant Mol Biol 34, 163–168 (1997). https://doi.org/10.1023/A:1005828325425

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