Abstract
Somatic embryogenesis was obtained from transverse thin cell layers (tTCLs) of Digitaria sanguinalis. tTCLs (0.2 - 0.4mm thick, 1mm in diameter) were excised from 4-week-old seedlings and placed onto Murashige and Skoog media supplemented with a varying concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) (from 1 µM to 100 µM) and sucrose (from 3% to 24%). Somatic embryos were obtained in the dark 7-10 days after inoculation from tTCLs excised at specific levels on the seedling and cultured in the presence of 2,4-D (5 µM to 10 µM) and sucrose (3 to 6%). The exposure of the tTCLs to light decreased the percentage of tTCLs forming somatic embryos. Viable plantlets were obtained 2 weeks after transfer onto a cytokinin-containing medium.
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Van Le, B., My Nghieng Thao, D., Gendy, C. et al. Somatic embryogenesis on Thin Cell Layers of a C4 species, Digitaria sanguinalis (L.) Scop. Plant Cell, Tissue and Organ Culture 49, 201–208 (1997). https://doi.org/10.1023/A:1005816126824
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DOI: https://doi.org/10.1023/A:1005816126824