Skip to main content

An efficient method for adventitious shoot regeneration from stem-segment explants of gypsophila

Abstract

An efficient adventitious shoot regeneration procedure was developed for Gypsophila paniculata L. Using cultivar Arbel, shoot regeneration from the three upper internodes of the stem was monitored on MS media supplemented with different cytokinins (thidiazuron, benzyladenine, kinetin or zeatin) and an auxin (naphthaleneacetic acid). Thidiazuron was found to be the most efficient cytokinin, with up to 100% of the explants forming shoots, at an average of up to 19 shoots per explant being regenerated. The highest percentage of shoot formation was observed in the stem explants originating from the first internode, with all cytokinins tested. The adventitious origin of shoots regenerated from stem explants was confirmed by scanning electron microscopy. The regeneration procedure was found to be applicable to five other gypsophila cultivars (Perfecta, Golan, Gilboa, Flamingo and Tavor). Regenerating plants were successfully transferred to soil, and did not differ in flower color, size or shape from standard vegetatively propagated plants derived from cuttings.

This is a preview of subscription content, access via your institution.

References

  1. Dillen W & Buysens S (1989) A simple technique to overcome vitrification in Gypsophila paniculata L. Plant Cell Tiss. Org. Cult. 19: 181–188

    Article  Google Scholar 

  2. Echlin P (1971) Preparation of labile biological material for examination in the scanning electron microscope. In: Heywood VH (ed) Scanning Electron Microscope (pp 307–315). Academic Press, New York

    Google Scholar 

  3. Han BH, Paek KY & Choi JK (1991) Micropropagation of Gypsophila paniculata using shoot tip culture in vitro. J. Korean Soc. Hort. Sci. 32: 394–400

    Google Scholar 

  4. Henry M (1993) Gypsophila paniculata L. (Baby's Breath): in vitro culture and the production of gypsogenin saponins. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 21 (pp 187–206). Springer-Verlag, Berlin, Heidelberg

    Google Scholar 

  5. Horsch R, Fraley R, Rogers S, Fry J, Klee H, Shah D, McCormick S, Niedermeyer J & Hoffmann N (1987) Agrobacterium-mediated transformation of plants. In: Green CE, Sommers DA, Hacket WP & Biesoer DD (eds) Plant Tissue and Cell Culture (pp 317–329). Alan Liss, New York

    Google Scholar 

  6. Huetteman CA & Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33: 105–119

    CAS  Article  Google Scholar 

  7. Kusey WE, Hammer PA & Weiler TC (1980) In vitro propagation of Gypsophila paniculata L. 'Bristol Fairy'. HortScience 15: 600–601

    Google Scholar 

  8. Lu C-Y, Nugent G & Wardly T (1990) Efficient, direct plant regeneration from stem segments of chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple). Plant Cell Rep. 8: 733–736

    CAS  Article  Google Scholar 

  9. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 357–366

    Article  Google Scholar 

  10. Nugent G, Richardson T & Lu C-Y (1991) Plant regeneration from stem and petal of carnation. Plant Cell Rep. 10: 477–480

    CAS  Article  Google Scholar 

  11. Pauthe-Dayde D, Rochd M & Henry R (1990) Triterpenoid saponin production in callus and multiple shoot cultures of Gypsophila spp. Phytochemistry 2: 483–487

    Google Scholar 

  12. Shillo R (1985) Gypsophila paniculata. In: Halevy AH (ed) Handbook of Flowering, Vol 3 (pp 83–87). CRC Press Inc, Boca Raton, Florida

    Google Scholar 

  13. Van Altvorst AC, Yancheva S & Dons H (1995) Cells within the nodal region of carnation shoots exhibit a high potential for adventitious shoot formation. Plant Cell Tiss. Org. Cult. 40: 51–57

    Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ahroni, A., Zuker, A., Rozen, Y. et al. An efficient method for adventitious shoot regeneration from stem-segment explants of gypsophila. Plant Cell, Tissue and Organ Culture 49, 101–106 (1997). https://doi.org/10.1023/A:1005806627306

Download citation

  • Caryophyllacae
  • cytokinins
  • t Gypsophila paniculata L.
  • internode
  • thidiazuron