Abstract
High-resolution solid-state 13C NMR spectra of the light-harvesting antenna complex (LH1) from Rhodospirillum rubrum were observed for the first time by cross-polarization (CP), magic angle spinning (MAS) methods with a total elimination of spinning side band technique (TOSS). Chemical shift analysis of the CP/MAS/TOSS 13C NMR spectra confirmed that the LH1 consists mainly of α-helices in the solid state. Time constants of cross polarization (TCH) and relaxation time T1 in a rotating frame (T1ρH) were determined from the experiments at various contact times. Smaller values of TCH were obtained for the carbons attached directly with protons in a rigid state. Relaxation times T1ρH revealed the dynamic structure of the complex and showed that bacteriochlorophyll a in the LH1 has high internal mobility even in the solid state. The proton spin-lattice relaxation time in a laboratory frame (T1H) determined by the 13C NMR signal amplitude changes suggested that protons in the LH1 proteins have such strong interaction among them that the spins of all protons in the protein can diffuse through spin-lattice-relaxation.
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Yoza, K., Wang, ZY., Kobayashi, M. et al. High-resolution solid-state 13C NMR of the LH1 from Rhodospirillum rubrum. Photosynthesis Research 52, 167–173 (1997). https://doi.org/10.1023/A:1005802117233
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DOI: https://doi.org/10.1023/A:1005802117233