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Lectin histochemistry of ethylnitrosourea-induced oligodendrogliomas in the rat

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Abstract

Oligodendrogliomas (n = 26) induced by ethylnitrosourea (ENU) in wistar rats were examined to assess the lectin specificity to oligodendroglial membranes. Two different types of oligodendrogliomas were found in our material: an isomorphous type (n = 12), and a polymorphous type (n = 14). The first one, with two variants according to its size, macro- (n = 9) and microtumors (n = 3), hadpredominantly a honey-comb pattern with ‘clear halos’ around the nuclei without anaplasia. The second type, composed mostly by macrotumours, was anaplastic, with high cellular density,necrosis and intratumoral hemorrhages. Peanut agglutinin (PNA) labelled plasma membranes of well-differentiated cellular components of the first group. The tumoral oligodendrocytes lost the property to bind PNA in the second group of tumours, while Concanavalin A (Con A) showed affinity to intracytoplasmic structures of these tumours. PNA is a reliable marker of oligodendroglial plasma membraneof well-differentiated ENU-induced oligodend rogliomas. This experimental model, using PNA and Con A, may have important clinical applications regarding the biological behaviourof this type of neoplasm.

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Author notes

  1. Javier Figols

    Present address: Department of Pathology, (Section of Neuropathology), Hospital Universitario ’Marqués de Valdecilla', 39008, Santander, Spain

Authors and Affiliations

  1. Institute of Neuropathology, Free University Berlin, Germany

    Javier Figols & Jorge Cervós-Navarro

  2. Department of Neuropathology, Medical Research Centre, Polish Acad. Sci, Warsaw, Poland

    Halina Kroh

  3. Neurological Tissue Bank, Hospital Clínico y Provincial, University of Barcelona, Spain

    Felix F. Cruz-Sánchez

Authors
  1. Javier Figols
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  2. Halina Kroh
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  3. Felix F. Cruz-Sánchez
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  4. Jorge Cervós-Navarro
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Figols, J., Kroh, H., Cruz-Sánchez, F.F. et al. Lectin histochemistry of ethylnitrosourea-induced oligodendrogliomas in the rat. J Neurooncol 31, 227–232 (1997). https://doi.org/10.1023/A:1005781811775

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