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Leaf senescence in Brassica napus: cloning of senescence related genes by subtractive hybridisation

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Abstract

A subtractive hybridisation technique was developed to clone cDNAs representing genes that showed enhanced expression during leaf senescence in Brassica napus. A number of different genes were identified that, when analysed by northern hybridisation, showed different patterns of expression during leaf development but were all expressed at increased levels during senescence. Sequence analysis of these cDNAs showed that several types of genes were found including two different proteases, glutamine synthetase, ATP sulphurylase, catalase, metallothionein, ferritin and an antifungal protein. The possible roles of these gene products in the senescence process are discussed.

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  1. Plant Molecular Biology Laboratory, Department of Biological Sciences, Wye College, University of London, Wye, Ashford, Kent, TN25 5AH, UK

    Vicky Buchanan-Wollaston & Charles Ainsworth

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  1. Vicky Buchanan-Wollaston
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  2. Charles Ainsworth
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Buchanan-Wollaston, V., Ainsworth, C. Leaf senescence in Brassica napus: cloning of senescence related genes by subtractive hybridisation. Plant Mol Biol 33, 821–834 (1997). https://doi.org/10.1023/A:1005774212410

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