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Restoration of TATA-dependent transcription in a heat-inactivated extract of tobacco nuclei by recombinant TATA-binding protein (TBP) from tobacco

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Abstract

We isolated a complementary DNA (cDNA) that encoded a TATA-binding protein (TBP) from a cDNA library of tobacco (Nicotiana tabacum) suspension-cultured cells (BY-2). A comparison among deduced amino acid sequences of plant TBPs revealed the presence of a long conserved region within the amino acid sequence of the TBP. Genomic Southern analysis revealed that tobacco TBP (tTBP) is encoded by only a small number of copies of a gene in the tobacco genome. Addition of recombinant tTBP to an extract of tobacco nuclei (TNE) enhanced the basal transcriptional activity in vitro. This result indicates that the level of tTBP is a rate-limiting factor for basal transcriptional activity in TNE. We subsequently succeeded in the functional complementation of TATA-dependent initiation of transcription that was associated with a plant promoter in a homologous plant system. Addition of bacterially expressed recombinant tTBP to a heat-inactivated TNE restored transcriptional activity, as did the addition of human TBP. Moreover, heating of the recombinant tTBP eliminated its ability to restore transcriptional activity. It appears that the heat inactivation of TNE was caused by the heat inactivation of tTBP in TNE.

Abbreviations: DTT, dithiothreitol; GTF, general transcription factor; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl sulfate; TBP, TATA-binding protein; TNE, extract of tobacco nuclei

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Authors and Affiliations

  1. Graduate School of Environmental Earth, Science, Hokkaido University, Sapporo, 060, Japan

    Ken-ichi Yamazaki

  2. Agricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-01, Japan

    Nobuko Iwataki, Akihiko Hoya & Ken-ichi Yamazaki

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  1. Nobuko Iwataki
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  2. Akihiko Hoya
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Iwataki, N., Hoya, A. & Yamazaki, Ki. Restoration of TATA-dependent transcription in a heat-inactivated extract of tobacco nuclei by recombinant TATA-binding protein (TBP) from tobacco. Plant Mol Biol 34, 69–79 (1997). https://doi.org/10.1023/A:1005759521285

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