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Expression and retinoid modulation of N-arginine dibasic convertase and an aminopeptidase-B in human neuroblastoma cell lines

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Abstract

Under retinoic acid exposure, the three SK-N-BE(2)-derived human neuroblastoma cell lines, BE(2)-NA, BE(2)-SA and BE(2)-M17 undergo mainly differentiation, apoptosis or continue to proliferate, respectively. We have used this model system to study the modulation of the transcriptional expression of putative processing enzymes, two novel metallopeptidases; i.e. N-arginine dibasic convertase (NRD convertase; EC 3.4.24.61) and an aminopeptidase-B after exposure of the cells either to retinoic acid or to synthetic retinoid analogs. The data indicate that the two respective enzymes are differently modulated in the various cell lines. Whereas aminopeptidase-B expression is enhanced in most cases, NRD convertase appears to undergo opposite regulation in proliferating versus differentiating neuroblastoma cells. It is concluded that both genes might contain retinoic acid regulatory elements (RARE) in their promoters.

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Authors and Affiliations

  1. Laboratoire de Biochimie des Signaux Régulateurs Cellulaires et Moléculaires, URA CNRS 1682, Université Pierre et Marie Curie, 96 Boulevard Raspail, 75006, Paris, France

    Muriel Draoui, Véronique Hospital, Sandrine Cadel, Thierry Foulon, Annik Prat, Nicole Barré & Paul Cohen

  2. IDI-IRCCS Biochemistry Laboratory at the Department of Experimental Medicine, University of Roma ‘Tor Vergata’, Italy

    Lorenza Bellincampi & Gerry Melino

  3. Department of Biology, University of L'Aquila, Italy

    Lorenza Bellincampi & Gerry Melino

  4. CIRD-Galderma, Sophia-Antipolis, France

    Uwe Reichert

Authors
  1. Muriel Draoui
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  2. Lorenza Bellincampi
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  3. Véronique Hospital
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  4. Sandrine Cadel
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  5. Thierry Foulon
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  6. Annik Prat
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  7. Nicole Barré
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  8. Uwe Reichert
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  9. Gerry Melino
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  10. Paul Cohen
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Draoui, M., Bellincampi, L., Hospital, V. et al. Expression and retinoid modulation of N-arginine dibasic convertase and an aminopeptidase-B in human neuroblastoma cell lines. J Neurooncol 31, 99–106 (1997). https://doi.org/10.1023/A:1005745717231

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  • DOI: https://doi.org/10.1023/A:1005745717231

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