Skip to main content
Log in

Cytokinins and cytokinin-binding sites in transgenic potato plants

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The introduction of the gene for cytokinin biosynthesis into the potato genome led to a manifold increase in the level of cytokinins (zeatin and zeatin riboside) in transgenic plants grown in vitro. The high amount of endogenous cytokinins in 20-day-old plants of clone 1339-3A correlated with high cytokinin-binding capacity of ribosomes that is presumably attribute to a cytokinin receptor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Brinegar AC (1994) Cytokinin binding proteins and receptors. In: Mok DWS and Mok MC (eds) Cytokinins: Chemistry, Activity, and Functions, pp 217–232. Boca Raton: CRC Press Inc

    Google Scholar 

  2. Davies JW and Cocking EC (1967) Protein synthesis in tomatofruit locule tissue. Incorporation of amino acids into protein by aseptic cell-free systems. Biochem J 104: 23–29

    Google Scholar 

  3. Fox JE and Erion JL (1975) A cytokinin binding protein from higher plant ribosomes. Biochem Biophys Res Commun 64: 694–700

    Google Scholar 

  4. Hamaguchi N, Iwamura H and Fujita T (1985) Fluorescent anticytokinins as a probe for binding: isolation of cytokinin-binding proteins from the soluble fraction and identification of a cytokinin-binding site on ribosomes of tobacco callus cells. Eur J Biochem 153: 565–572

    Google Scholar 

  5. Jayabaskaran C (1990) Isolation and characterization of a cytokinin-binding protein from cucumber cotyledons. Plant Growth Regul 9: 9–17

    Google Scholar 

  6. Klyachko NL, Ananiev E and Kulaeva ON (1979) Effect of 6-benzylaminopurine and abscisic acid on protein synthesis in detached pumpkin cotyledons. Physiol Veg 17: 607–617

    Google Scholar 

  7. Klyachko NL, Yakovleva LA, Shakirova FM and Kulaeva ON (1982) Cell-free translation of polyribosomes from detached pumpkin cotyledons: effects of starvation and cytokinin. Biol Plant 24: 374–380

    Google Scholar 

  8. Kulaeva ON, Karavaiko NN, Moshkov IE, Selivankina SYu and Novikova GV (1990) Isolation of a protein with cytokinin-receptor properties by means of anti-idiotype antibodies. FEBS Lett 261: 410–412

    Google Scholar 

  9. Mitsui S and Sigiura M (1993) Purification and properties of cytokinin-binding proteins from tobacco leaves. Plant Cell Physiol 34: 543–547

    Google Scholar 

  10. Momotani E and Tsuji H (1992) Isolation and characterization of a cytokinin-binding protein from the water-soluble fraction of tobacco leaves. Plant Cell Physiol 33: 407–412

    Google Scholar 

  11. Moore III FH (1979) A cytokinin-binding protein from wheat germ. Isolation by affinity chromatography and properties. Plant Physiol 64: 594–599

    Google Scholar 

  12. Ondrej M, Hrouda M, Karavaiko NN, Matousek J, Mikulovich TP, Pavingerova D and Vlasak J (1989) Transformation by Agrobacterium vectors and the study of function of plant hormones. In: Krekule J and Seidlova F (eds) Signals in Plant Development, pp 73–89. The Hague: SPB Academic Publishing

    Google Scholar 

  13. Polya GM and Davis AW (1978) Properties of a high affinity cytokinin-binding protein from wheat germ. Planta 139: 139–147

    Google Scholar 

  14. Yakovleva LA and Kulaeva ON (1987) The effect of phytohormones on phosphorylation of ribosomal proteins in detached pumpkin cotyledons. Biochem Physiol Pflanzen 182: 359–365

    Google Scholar 

  15. Yakovleva LA, Klueva NY and Kulaeva ON (1992) Phosphorylation of ribosome proteins as a mechanism of phytohormone regulation of protein synthesis in plants. In: Krekule J and Seidlova F (eds) Signals in Plant Development, pp 169–172. The Hague: SPB Academic Publishing

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yakovleva, L., Cheredova, E. & Karavaiko, N. Cytokinins and cytokinin-binding sites in transgenic potato plants. Plant Growth Regulation 21, 71–73 (1997). https://doi.org/10.1023/A:1005745510883

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005745510883

Navigation