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Folate receptors as potential therapeutic targets in choroid plexus tumors of SV40 transgenic mice

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Abstract

A high affinity folate receptor is expressed in some human cancers,including choroid plexus tumors and ependymomas, and has been suggested as a target for therapeutics. In this report, the expression of folate receptors in an SV40 large T antigen transgenicmouse (SV11) was investigated. SV11 mice develop choroid plexus tumors, a property that may be related to the observation that SV40 has been isolated from human choroid plexus tumors and ependymomas. We report that SV11 choroid plexus tumors containa high affinity folate receptor (D of 1 nM), detectable by 125I-folate autoradiography and immunohistochemistry.Western blot analysis indicated an apparent molecular weight of 38 kDa.RT-PCR revealed the presence of transcripts for bothalpha and beta isoforms of the folate receptor. Brain parenchyma hasundetectable folate receptor, but normal choroid plexus has substantiallevels (as does human choroid plexus). The folate receptors of the tumorare accessible from the bloodstream whereasthose of the normal choroid plexus are not. Thus SV11 transgenic mice should be useful for evaluating therapeutic targeting of high affinity folate receptors, both for efficacy of specific agents andpossible side effects.

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Authors and Affiliations

  1. Neuroscience Program, USA

    Todd A. Patrick & Edward J. Roy

  2. Department of Biochemistry, USA

    David M. Kranz & Edward J. Roy

  3. Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC, 27599, USA

    Terry A. van Dyke

  4. Department of Psychology, University of Illinois, Urbana, IL, 61801

    Edward J. Roy

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  1. Todd A. Patrick
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  2. David M. Kranz
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  3. Terry A. van Dyke
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  4. Edward J. Roy
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Patrick, T.A., Kranz, D.M., Dyke, T.A.v. et al. Folate receptors as potential therapeutic targets in choroid plexus tumors of SV40 transgenic mice. J Neurooncol 32, 111–123 (1997). https://doi.org/10.1023/A:1005713115147

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