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Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

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Abstract

A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.

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Authors and Affiliations

  1. Department of Cellular Biology, Physiology and Immunology, Faculty of Medicine, Universitat Autònoma de Barcelona, 08193, Bellaterra (Barcelona), Spain

    Montserrat Solanas, Raquel Moral & Eduard Escrich

Authors
  1. Montserrat Solanas
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  2. Raquel Moral
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  3. Eduard Escrich
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Correspondence to Eduard Escrich.

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Solanas, M., Moral, R. & Escrich, E. Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues. Biotechnology Letters 23, 263–266 (2001). https://doi.org/10.1023/A:1005693928291

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  • DOI: https://doi.org/10.1023/A:1005693928291

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