Skip to main content
SpringerLink
Log in

UDP-Glucuronosyltransferase activity is correlated to saponin production in Gypsophila paniculata root in vitro cultures

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

UDP-Glucuronosyltransferase (UGT, EC 2.4.1.17) activity was detected in excised root cultures of Gypsophila paniculata. The UGT activity (up to 0.8 nkat mg−1 protein) correlated to the total saponin content (2 mg g−1 dw) during the exponential phase of the batch culture. This gives rise to the hypothesis of a direct relationship in the biosynthetic pathway regulation between the enzyme activity and the saponin biosynthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anhalt S, Weissenböck G ( 1992) Subcellular localization of luteolin glucuronides and related enzymes in rye mesophyll. Planta 187: 83-88.

    Google Scholar 

  • Baydoun EA, Waldron KW, Brett CT (1989) The interaction of xylosyltransferase and glucuronyltransferase involved in glucuronoxylan synthesis in pea (Pisum sativum) epicotyls. Biochem J. 257: 853-858.

    Google Scholar 

  • Colin-Neiger A, Kauffman I, Boutin JA, Fournel S, Siest G, Batt AM, Magdalou J (1984) Assessment of the Mulder and Van Doorn kinetic procedure and rapid centrifugal analysis of UDPglucuronosyltransferase activities. J. Biochem. Biophys. Meth. 9: 69-79.

    Google Scholar 

  • Earle ED, Langhans RW (1975) Carnation propagation from shoot tips cultured in liquid medium. Hortscience 10: 608-610.

    Google Scholar 

  • Fulcheri C, Morard P, Henry M (1998) Stimulation of the growth and the triterpenoid saponin accumulation of Saponaria officinalis cell and Gypsophila paniculata root suspension cultures by improvement of the mineral composition of the media. J. Agric. Food Chem. 46: 2055-2061.

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158.

    Google Scholar 

  • Henry M, Pauthe-Dayde D, Rochd M (1989) Extraction and quantitative HPLC determination of Gypsogenin 3,0-glucuronide. J. Chromatogr. 477: 413-419.

    Google Scholar 

  • Kauss H (1967) Biosynthesis of the glucuronic acid unit of hemicellulose B from UDP-glucuronic acid. Biochim. Biophys. Acta 148: 572-574.

    Google Scholar 

  • Mackenzie PI, Owens IS, Burchell B, Bock KW, Bairoch A, Belanger A, Fournel-Gigleux S, Green M, Hum DW, Iyanagi T, Lancet D, Louisot P, Magdalou J, Chowdbury JR, Ritter JK, Schachter H, Tephly TR, Tipton KF, Nebert DW (1997) The UDP-glycosyltransferase gene superfamily: recommended nomenclature update based on evolutionary divergence. Pharmacogenetics 7: 255-269.

    Google Scholar 

  • Verpoorte R, van der Heijden R, ten-Hoopen HJG, Memelink J (1999) Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals. Biotechnol. Lett. 21: 467-479.

    Google Scholar 

  • Waldron KW, Brett CT (1983) A glucuronyltransferase involved in glucuroxylan synthesis in pea (Pisum sativum) epicotyls. Biochem. J. 213: 115-22.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculté de Pharmacie, Université Henri Poincaré, Nancy I, 5, Rue Albert-Lebrun, B.P. 403, 54001, Nancy Cedex, France

    Marie-Claire Hérold & Max Henry

Authors
  1. Marie-Claire Hérold
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Max Henry
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Max Henry.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hérold, MC., Henry, M. UDP-Glucuronosyltransferase activity is correlated to saponin production in Gypsophila paniculata root in vitro cultures. Biotechnology Letters 23, 335–337 (2001). https://doi.org/10.1023/A:1005689104107

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005689104107

Search

Navigation