Abstract
Two genes, xynA and xynB, encoding xylanases from Paenibacillus sp. KCTC 8848P were cloned and expressed in Escherichia coli, and their nucleotide sequences were determined. The xylanases of E. coli transformants were released into the extracellular culture fluid in the absence of xylan. The structural gene of xynA 636 bp, encoded a protein of 212 amino acids, while the xynB gene consisted of 951 bp open reading frame for a protein of 317 amino acids. The amino acid sequence of the xynAgene showed 83% similarity to the xylanase of Aeromonas caviae, and belonged to the family 11 glycosyl hydrolases. The deduced amino acid sequence of the xynB gene, however, showed 51% similarity to the xylanase of Rhodothermus marinus, and belonged to the family 10 glycosyl hydrolases.
Similar content being viewed by others
References
Biely P (1985) Microbial xylanolytic systems.Trends Biotechnol. 3: 286-290.
Cazemier AE, Verdoes JC, van Ooyen AJJ, den Camp HJMO (1999) Molecular and biochemical characterization of two xylanaseencoding genes from Cellulomonas pachnodae. Appl. Environ. Microbiol. 65: 4099-4107.
Cho SG, Choi YJ (1995) Nucleotide sequence analysis of an endo-xylanase gene (xynA) from Bacillus stearothermophilus. J. Microbiol. Biotechnol. 5: 117-124.
Gat O, Lapidot A, Alchanati I, Regueros C, Shoham Y (1994) Cloning and DNA sequence of the gene coding for Bacillus stearothermophilus T-6 xylanase. Appl. Environ. Microbiol. 60: 1889-1896.
Gilkes NR, Henrissat B, Kilburn DG, Miller RC, Warren RAJ (1991) Domains in microbial β-1,4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev. 55: 303-315.
Khasin A, Alchanati I, Shoham Y (1993) Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6. Appl. Environ. Microbiol. 59: 1725-1730.
Lopez C, Blanco A, Pastor FIJ (1998) Xylanase production by a new alkali-tolerant isolate of Bacillus. Biotechnol. Lett. 20: 243-246.
MacKenzie CR, Bilous D, Schneider H, Johnson KG (1987) Induction of cellulolytic and xylanolytic enzyme systems in Streptomyces spp. Appl. Environ. Microbiol. 53: 2835-2839.
Morosoli R, Shareck F, Moreau A, Kluepfel D (1990) Expression of xylanase genes in Streptomyces lividans. In: Heslot H, Davies J, Florent J, Bobichon L, Durand G, Penasse L, eds. Proceedings of 6th International Symposium of Genetics of Industrial Microorganisms. Strasbourg, France: Société Française de Microbiologie, pp. 935-946.
Morosoli R, Zalce E, Durand S (1993) Secretion of a Cryptococcus albidus xylanase in Pichia stipitis resulting in a xylan fermenting transformant. Curr. Genet. 24: 94-99.
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res. 8: 4321-4325.
Sanger F, Niklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467.
von Heijne G (1986) A new method for predicting signal sequence cleavage sites. Nucl. Acids Res. 14: 4683-4690.
Wong KKY, Tan LUL, Saddler JN (1988) Multiplicity of β-1,4-xylanase in microorganisms: functions and applications. Microbiol. Rev. 52: 305-317.
Walsh DJ, Bergquist PL (1997) Expression and secretion of a thermostable bacterial xylanase in Kluyveromyces lactis. Appl. Environ. Microbiol. 63: 3297-3300.
Yang RCA, MacKenzie CR, Bilous D, Seligy VL, Narang SA (1988) Molecular cloning and expression of a xylanase gene from Bacillus polymyxa in Escherichia coli. Appl. Environ. Microbiol. 54: 1023-1029.
Yang RCA, MacKenzie CR, Bilous D, Narang SA (1989) Identifi-cation of two distinct Bacillus circulans xylanases by molecular cloning of the genes and expression in Escherichia coli. Appl. Environ. Microbiol. 55: 568-572.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, HJ., Shin, DJ., Cho, N.C. et al. Cloning, expression and nucleotide sequences of two xylanase genes from Paenibacillus sp.. Biotechnology Letters 22, 387–392 (2000). https://doi.org/10.1023/A:1005676702533
Issue Date:
DOI: https://doi.org/10.1023/A:1005676702533