Abstract
Polyethylenimine (PEI) interferes strongly in all commonly employed protein estimation assays. Here, a strategy to remove this interference is developed. Polyethylenimine is selectively removed by adsorption from a mixture containing PEI and lysozyme using carboxymethyl-cellulose, a cation-exchange resin between an ionic strength of 0.35–1 M (pH 6.0), conditions under which lysozyme remains in solution and can be accurately estimated.
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Gupta, V., Nath, S. & Chand, S. Estimation of proteins in the presence of polyethylenimine. Biotechnology Letters 22, 927–929 (2000). https://doi.org/10.1023/A:1005676531595
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DOI: https://doi.org/10.1023/A:1005676531595