Abstract
A flow injection system for the on-line detection of the intracellular enzyme lactate dehydrogenase (LDH) during fermentation has been developed. The system is comprised of an on-line cell disintegration part, an immobilised dye based expanded bed column for the affinity capture of LDH and a fluorimetric detection unit. The system with a linearity of 0.1–5.4 U LDH ml−1 was applied for the detection of intracellular accumulation of LDH during Lactococcus lactis subsp.lactis cultivation.
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Nandakumar, M., Nandakumar, R. & Mattiasson, B. Fluorimetric quantification of intracellular lactate dehydrogenase during fermentation using flow injection analysis. Biotechnology Letters 22, 1453–1457 (2000). https://doi.org/10.1023/A:1005672817354
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DOI: https://doi.org/10.1023/A:1005672817354