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The exposure to trans-cinnamic acid of osmotically stressed Catharanthus roseus cells cultured in a 14-l bioreactor increases alkaloid accumulation

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Abstract

A Catharanthus roseus cell line was cultured in a 14-l bioreactor. Total alkaloid production decreased more than 80% while scaling up this cell line from 250 ml batch cultures to the bioreactor. However, the subsequent application of an osmotic stress and 1 mM trans-cinnamic acid, which inhibits the synthesis of phenolic compounds, restored the original alkaloid amounts.

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Authors and Affiliations

  1. Unidad de Biología Experimental, Centro de Investigación Científica de Yucatán, A.C. Calle 43

    Gregorio C. Godoy-Hernández, Felipe A. Vázquez-Flota & Víctor M. Loyola-Vargas

  2. No. 130 Col. Chuburna de Hidalgo, C.P. 97200, Mérida Yucatán, México

    Gregorio C. Godoy-Hernández, Felipe A. Vázquez-Flota & Víctor M. Loyola-Vargas

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  1. Gregorio C. Godoy-Hernández
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  2. Felipe A. Vázquez-Flota
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  3. Víctor M. Loyola-Vargas
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Godoy-Hernández, G.C., Vázquez-Flota, F.A. & Loyola-Vargas, V.M. The exposure to trans-cinnamic acid of osmotically stressed Catharanthus roseus cells cultured in a 14-l bioreactor increases alkaloid accumulation. Biotechnology Letters 22, 921–925 (2000). https://doi.org/10.1023/A:1005672400219

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