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Stabilization of papain and lysozyme for application to cosmetic products

Biotechnology Letters volume 22, pages 137–140 (2000)Cite this article

Abstract

For cosmetic applications, papain or lysozyme was conjugated to a soluble biopolymer produced by Schizophyllum commune. Stability of the conjugated enzymes were significantly enhanced, such that more than 90% of the initial activity remained after a month storage at 45 °C, even in a cosmetic formulation including various oils and surfactants. Cosmetic lotion containing 1% papain conjugate was more effective in exfoliating stratum corneum of skin than the lotion containing 5% lactic acid, one of the popular exfoliating agents.

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References

  • Besson C, Favrebonvin G, Ofagain C, Wallach J (1995) Chemical derivatives of Pseudomonas aeruginosa elastase showing increased stability. Enzyme Microbiol. Technol. 17: 877–881.

    Google Scholar 

  • Efendiev AI, Tolstykh PI, Dadashev AI, Marshava OM (1991) Prolonged local enzyme therapy of suppurative wounds. Khirurgiia (Moscow) 7: 48–50.

    Google Scholar 

  • Forestier JP (1992) Les enzymes de l'espace extracellulaire du stratum corneum. Int. J. Cosmetic Sci. 14: 47–55.

    Google Scholar 

  • Kim NS, Kim SI (1994) Characterization and primary specificity of an extracellular metalloproteinase from Serratia marcescens. Can. J. Microbiol. 40: 120–126.

    Google Scholar 

  • Pierard GE, Pierard-Franchimont C (1993) Dihydroxyacetone test as a substitute for the dansyl chloride test. Dermatology 186: 133–137.

    Google Scholar 

  • Shugar D (1952) The measurement of lysozyme activity and the ultraviolet inactivation of lysozyme. Biochim. Biophys. Acta 8: 302–309.

    Google Scholar 

  • Sundaram PV, Venkatesh R (1998) Retardation of thermal and urea induced inactivation of alpha-chymotrypsin by modification with carbohydrate polymers. Protein Eng. 11: 699–705.

    Google Scholar 

  • Takaoka T, Katsuta H, Ishiki S, Konishi T (1972) Effects of lysozyme on the proliferation of fibroblasts in tissue culture. Japan J. Exp. Med. 42: 221–232.

    Google Scholar 

  • Wenninger J, McEwen GN (1997) International Cosmetic Ingredient Dictionary and Handbook, 7th edn. Washington: CTFA.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Pacific Corporation /, R&D Center, Bora-ri 314-1, Kiheung-eup, Yongin-si, Kyounggi-do, 449-900, Republic of Korea

    Young-Chul Sim, Sung-Gu Lee, Dong-Cheol Lee, Byung-Young Kang, Kyung-Mok Park, Jang-Young Lee, Moo-Sung Kim & Ih-Seop Chang

  2. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Kusong-dong 373-1, Yusong-gu, Taejon, 305-701, Republic of Korea

    Joon-Shick Rhee

Authors
  1. Young-Chul Sim
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  2. Sung-Gu Lee
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  3. Dong-Cheol Lee
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  4. Byung-Young Kang
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  5. Kyung-Mok Park
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  6. Jang-Young Lee
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  7. Moo-Sung Kim
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  8. Ih-Seop Chang
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  9. Joon-Shick Rhee
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Sim, YC., Lee, SG., Lee, DC. et al. Stabilization of papain and lysozyme for application to cosmetic products. Biotechnology Letters 22, 137–140 (2000). https://doi.org/10.1023/A:1005670323912

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  • DOI: https://doi.org/10.1023/A:1005670323912

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