Abstract
Lipase from Nigella sativa seeds was immobilized by adsorption on Celite 535 from phosphate buffer solutions varying pH values of 5.0–8.0 at 25 °C. Langmuir isotherms described the adsorption equilibria well for lipase adsorption at all pH range. The saturation capacity for adsorption of lipase increased from 14.5 to 24.3 mg g−1 Celite as the adsorption pH was reduced from 8 to 5, but the adsorption equilibrium constant remained constant and was determined to be 1.92 × 105 M−1. The adsorbed enzymes showed different activity values depending on the pH of the adsorption medium. The immobilized enzymes prepared at pH 6 displayed the highest activity values.
Similar content being viewed by others
References
Bosley JA, Peilow AD (1997) Immobilization of lipases on porous polypropylene: reduction in esterification efficiency at low loading. J. Amer. Oil Chem. Soc. 74: 107-111.
Chaplin MF, Bucke C (1990) Enzyme Technology. Cambridge: Cambridge University Press, p. 16.
Cui YM, Wei DZ, Yu JT (1997) Lipase-catalyzed esterification in organic solvent to resolve racemic naproxen. Biotechnol. Lett. 19: 865-868.
Dandik L, Aksoy HA (1996) Application of Nigella sativa seed lipase in oleochemical reactions. Enzyme Microb. Technol. 19: 277-281.
Gonçalves APV, Lopes JM, Lemos F, Ribeiro FR, Prazeres DMF, Cabral JMS, Aires-Barros MR (1997) Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi. Enzyme Microb. Technol. 20: 93-101.
Karakazan S (1997) Corekotu (Nigella sativa L.) tohumlarindan lipaz enziminin ekstraksiyonu ve karakterizasyonu [Extraction and characterization of lipase from black cumin (Nigella sativa L.) seeds]. M. Sci. Thesis, Istanbul Technical University, Chemical Engineering Department, Istanbul (in Turkish).
Kerzel P, Wenzig E, Mersmann A (1992) Investigation of the influence of several parameters on the adsorption of lipase. In: DECHEMA Biotechnology Conferences 5. VCH Verlagsgesellschaft, pp. 653-657.
Lee KT, Akoh CC (1998) Structured lipids: synthesis and applications. Food Rev. Int. 14: 17-34.
Li ZY, Ward OP (1993) Enzyme catalyzed production of vegetable oils containing omega-3 polyunsaturated fatty acids. Biotechnol. Lett. 15: 185-188.
Malcata FX, Reyes HR, Garcia HS, Hill CG, Amundson CH (1990) Immobilized lipase reactors for modification of fats and oils. A review. J. Amer. Oil Chem. Soc. 67: 890-910.
Rajendran S, Sudhindra Rao K, Prakash V (1990) Effect of pH in the acidic region on the structural integrity of lipase from wheat germ. Indian J. Biochem. Biophys. 27: 300-310.
Reetz MT, Zonda A, Simpelkamp J (1996) Efficient immobilization of lipases by entrapment in hydrophobic sol-gel materials. Biotechnol. Bioeng. 49: 527-534.
Ustun G, Turkay S, Karaali A (1998) Nigella sativa seeds: a potential source for oils and oleochemicals. In: Koseoglu SS, Rhee KC, Wilson RF, eds. Proceedings of the World Conference on Oilseed and Edible Oils Processing, Vol. 2. Champaign: AOCS Press, pp. 155-160, ISBN 0-935315-84-2.
Warmuth W, Wenzig E, Mersmenn A (1995) Selection of a support for immobilization of a microbial lipase for the hydrolysis of triglycerides. Bioprocess Eng. 12: 87-93.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Akova, A., Ustun, G. Activity and adsorption of lipase from Nigella sativa seeds on Celite at different pH values. Biotechnology Letters 22, 355–359 (2000). https://doi.org/10.1023/A:1005668500716
Issue Date:
DOI: https://doi.org/10.1023/A:1005668500716