Abstract
Bixin, a carotenoid, was esterified by l-ascorbic acid using immobilized lipase B from Candida antarctica. The conversion of l-ascorbic acid was 25% under atmospheric pressure. Ester production was twice higher when working under reduced pressure and was third lower when norbixin was used as a substrate-acyl donor due to lipase specificity, leading to a conversion of l-ascorbic acid of 50% and 8%, respectively.
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Humeau, C., Rovel, B. & Girardin, M. Enzymatic esterification of bixin by l-ascorbic acid. Biotechnology Letters 22, 165–168 (2000). https://doi.org/10.1023/A:1005665913685
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DOI: https://doi.org/10.1023/A:1005665913685