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Expression of Bacillus sp. β-xylosidase gene (xylB) in Saccharomyces cerevisiae

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Abstract

β-Xylosidase gene (xylB) from Bacillus sp. was amplified and inserted between GAL10 promoter and GAL7 terminator. For the secretory production of xylB in Saccharomyces cerevisiae, in-frame fusion of the exoinulinase signal sequence (INU1s) of Kluyveromyces marxianus to the upstream of xylB was conducted. When a transformant of S. cerevisiae harboring the resulting plasmid was grown on galactose-containing medium, most of β-xylosidase activity was localized in the periplasmic space of yeast and a maximum total activity reached about 2.9 unit ml−1 at 42 h cultivation. The recombinant β-xylosidase was produced as an active dimer form.

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Authors and Affiliations

  1. Department of Microbiology, Dong-Eui University, Pusan, 614-714, Korea

    Jong-Hyun Kim, Byung-Woo Kim & Soo-Wan Nam

  2. School of Food Biotechnology, Woosong University, Taejon, 300-100, Korea

    Ki-Hong Yoon

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  1. Jong-Hyun Kim
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  2. Byung-Woo Kim
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  3. Ki-Hong Yoon
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  4. Soo-Wan Nam
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Kim, JH., Kim, BW., Yoon, KH. et al. Expression of Bacillus sp. β-xylosidase gene (xylB) in Saccharomyces cerevisiae. Biotechnology Letters 22, 1025–1029 (2000). https://doi.org/10.1023/A:1005661909095

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