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Reactivation of Horseradish peroxidase in organic solvent using extraction

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Abstract

A novel extraction method was developed aiming at increasing the stability of enzymes in organic solvent media. Horseradish peroxidase (HRP), inactivated in a tetrahydrofuran (THF)/water (1:1, v/v), regained and maintained its activity when HRP was extracted by adding a THF/benzene mixture to the original solution. However, the HRP activity was drastically lowered in the enzyme-free blank solution that had been formed by employing the same extraction procedure. As a result, the reactivation after the extraction is believed to depend on enzyme history, and might be arisen from an irreversible structural change of the enzyme.

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References

  • Adams GDJ, Ramsay JR (1996) Optimizing the lyophilization cycle and the consequences of collapse on the pharmaceutical acceptability of erwinia l-asparaginase. J. Pharm. Sci. 85: 1301–1305.

    Google Scholar 

  • Griebenow K, Klibanov AM (1996) On protein denaturation in aqueous-organic mixtures but not in pure organic-solvents. J. Am. Chem. Soc. 118: 11695–11700.

    Google Scholar 

  • Herskovits TT, Gadegbeku B, Jaillet H (1970) On the structural stability and solvent denaturation of proteins. J. Biol. Chem. 245: 2588–2598.

    Google Scholar 

  • Joo H, Yoo YJ, Dordick JS (1998) Polymers from biocatalysts. Korean J. Chem. Eng. 15: 362–374.

    Google Scholar 

  • Joo H, Yoo YJ, Ryu DDY (1996) A biosensor stabilized by polyethylene-glycol for the monitoring of hydrogen-peroxide in organic-solvent media. Enzyme Microbiol. Tech. 19: 50–56.

    Google Scholar 

  • Klibanov AM (1986) Enzymes that work in organic solvents. Chemtechnology 16: 354–359.

    Google Scholar 

  • Klibanov AM (1997) Why are enzymes less active in organicsolvents than in water. Trends Biotechnol. 15: 97–101.

    Google Scholar 

  • Ryu K, Dordick JS (1992) How do organic solvents affect peroxidase structure and function. Biochemistry 31: 2588–2598.

    Google Scholar 

  • Ueji S, Fujino R, Okubo N, Miyazawa T, Kurita S, Kitadani M, Muromatsu A (1992) Solvent-induced inversion of enantioselectivity in lipase-catalyzed esterification of 2-phenoxypropionic acids. Biotechnol. Lett. 14: 163–168.

    Google Scholar 

  • Wetlaufer DB (1962) Ultraviolet spectra of proteins and amino acids. Adv. Protein Chem. 17: 303–390.

    Google Scholar 

  • Zaks A, Klibanov AM (1988) The effect of water on enzyme action in organic media. J. Biol. Chem. 263: 3194–3201.

    Google Scholar 

Download references

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Authors and Affiliations

  1. School of Chemical Engineering, Seoul National University, Seoul, 151-742, Korea

    Jin Ryoun Kim, Yoo Seong Choi & Young Je Yoo

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  1. Jin Ryoun Kim
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  2. Yoo Seong Choi
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  3. Young Je Yoo
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Kim, J.R., Choi, Y.S. & Yoo, Y.J. Reactivation of Horseradish peroxidase in organic solvent using extraction. Biotechnology Letters 22, 485–489 (2000). https://doi.org/10.1023/A:1005660131552

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  • DOI: https://doi.org/10.1023/A:1005660131552

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