Skip to main content
SpringerLink
Log in

Fourier-transformed infrared assay for the determination of the positional specificity of non-specific and 1,3-specific lipases

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A new fast infrared spectroscopy method for determination of the positional specificity of the lipase reaction is reported. This method is based on the analysis of specific infrared bands shown by the ester (1751 cm−1) and thioester (1704 cm−1) groups of the 2,3-dimercapto-1-propanol tributyrate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bereuter TL, Lorbeer E (1995) Monitoring of lipase-catalyzedcleavage of acylglycerols by high-temperature gas chromatography.J. Chromatogr. 697: 469–474.

    Google Scholar 

  • Commenil P, Belingheri L, Sancholle M, Dehorter B (1995) Purification and properties of an extracellular lipase from the fungus Botrytis cinerea.Lipids 30: 351–356.

    Google Scholar 

  • Ergan F, Andre G (1989) Simple high performance liquid chromatography methods for monitoring lipase reactions.Lipids 24:76–78.

    Google Scholar 

  • Farias RN, Torres M, Canela R (1997) Spectrophotometric determination of the positional specificity of nonspecific and 1,3-specificlipases.Anal. Biochem. 252: 186–189.

    Google Scholar 

  • Kovac A, Stadler P, Haalck L, Spener F, Paltauf F (1996) Hydrolysisand esterification of acylglycerols and analogs in aqueous medium catalyzed by microbial lipases.Biochim. Biophys. Acta 1301: 57–66.

    Google Scholar 

  • Li-Zu-Yi, Ward OP (1993) Lipase-catalyzed esterification of glyceroland n-3 polyunsaturated fatty acid concentrate in organic solvent.J. Am. Oil. Chem. Soc. 70: 745–748.

    Google Scholar 

  • Mozaffar Z, Weete JD (1993) Purification and properties of an extracellular lipase from Pythium ultimum.Lipids 28: 377–382.

    Google Scholar 

  • O'Connor CJ, Cleverly DR (1994) Fourier-transform infrared assay of bile salt-stimulated lipase activity in reversed micelles.J.Chem. Tech. Biotechnol. 61: 209–214.

    Google Scholar 

  • Uzawa H, Ohrui H, Meguro H, Mase T, Ichida A (1993) A convenientevaluation of the stereoselectivity of lipase-catalyzedhydrolysis of tri-O-acylglycerols on a chiral-phase liquid chromatography.Biochim. Biophys. Acta 1196: 165–168.

    Google Scholar 

  • Veeraragavan K (1990) A simple and sensitive method for theestimation of microbial lipase activity.Anal. Biochem. 186:301–305.

    Google Scholar 

  • Walde P, Luisi PL (1989) A continuous assay for reverse micellesbased on Fourier transform infrared spectroscopy.Biochemistry 28: 3353–3360.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (CONICET-UNT) and Instituto de Química Biológica Dr. Bernabé Bloj, Chacabuco 461, 4000, San Miguel de Tucumán, Tucumán, Argentina

    Ricardo N. Farias & Rosana N. Chehin

  2. Chemistry Department, Universitat de Lleida, Rovira Roure, 177, 25198-, Lleida, Spain

    Ramon Canela

Authors
  1. Ricardo N. Farias
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rosana N. Chehin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ramon Canela
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Farias, R.N., Chehin, R.N. & Canela, R. Fourier-transformed infrared assay for the determination of the positional specificity of non-specific and 1,3-specific lipases. Biotechnology Letters 22, 905–908 (2000). https://doi.org/10.1023/A:1005656012514

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005656012514

Search

Navigation