Dibutyl phthalate, oleic acid and terpineol were used to extract paclitaxel in situ fromTaxus chinensis suspension cultures. Oleic acid/terpineol (1:1, v/v) added to the cultures gave a higher paclitaxel concentration, compared with either of them alone. Oleic acid/terpineol (1:1, v/v) incorporated into the cultures at 3:50 (v/v) 4 days after elicitation, which was carried out by adding 50 mg chitosan l−1, 60 μM methyl jasmonate and 30 μM Ag+ to 10-day-old cultures, resulted in the greatest paclitaxel production of 48 mg l−1 at day 10 after elicitation. This was double that of the culture by elicitation, and 7-fold higher than that of the culture by in situ extraction.
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Zhang, CH., Xu, HB. Improved paclitaxel production by in situ extraction and elicitation in cell suspension cultures of Taxus chinensis. Biotechnology Letters 23, 189–193 (2001). https://doi.org/10.1023/A:1005655219649
- in situ extraction
- paclitaxel (TaxolR)
- plant cell culture
- Taxus chinensis