Abstract
Various used paper materials have been exposed to the action of cellulases from Penicillium funiculosum, Trichoderma reesei, Trichoderma viride and Aspergillus niger. A 2 h incubation period showed cellulase from T. viride the most active except for office paper that was maximally degraded by A. niger cellulase. Cellulase mixtures increased saccharification while sequential treatment with cellulases from T. reesei and P. funiculosum increased biodegradation at values between 15% and 190%. The maximum increase of saccharification (190%) was obtained when T. reesei cellulase initiated the sequential treatment of newspaper relative to the sole action of P. funiculosum cellulase on this non-pretreated and pretreated material.
Similar content being viewed by others
References
Agunwamba JC (1998) Solid waste management in Nigeria: problems and issues. Environ. Manage. 22: 849–856.
Block D (1999) Executive order and proposed bill will boost biobased products and bioenergy. Biocycle Magazine: 59–61.
Gupta V (1998) Where now for wastepaper? Paper Asia. 14: 26–28.
Jeffries TW, Schartmann R (1999) Bioconversion of secondary fiber fines to ethanol using counter-current enzymatic saccharification and co-fermentation. Appl. Biochem. Biotechnol. 77: 435–444.
Miller GL (1959) Use of dinitrosalicylic acid (DNS) for determination of reducing sugars. Anal. Chem. 31: 426–428.
Nguyen QA, Keller FA, Tucker MP, Lombard CK, Jenkins BM, Yomogida DE, Tiangco VM (1999) Bioconversion of mixed solids waste to ethanol. Appl. Biochem. Biotechnol. 77: 455–471.
Schmidt S, Padukone N (1997) Production of lactic acid from wastepaper as a cellulosic feedstock. J. Indust. Microbiol. Biotechnol. 18: 10–14.
Van Wyk JPH (1998) Paper hydrolysis by cellulase from Penicillium funiculosum and Trichoderma viride. Biores. Technol. 63: 275–277.
Van Wyk JPH (1999) Saccharification of paper products by cellulase from Penicillium funiculosum and Trichoderma reesei. Biomass Bioenergy 16: 239–242.
Van Wyk JPH, Botha AC (1997) Hydrolysis of cellulose materials during successive treatment with cellulase from Penicillium funiculosum. Biotechnol. Lett. 19: 687–689.
Van Wyk JPH, Mogale AM (1998) Saccharification of paper mateials by mixtures of cellulase from Penicillium funiculosum and Aspergillus niger. Australasian Biotechnol. 8: 357–359.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van Wyk, J., Mogale, M. & Seseng, T. Saccharification of used paper with different cellulases. Biotechnology Letters 22, 491–494 (2000). https://doi.org/10.1023/A:1005648316531
Issue Date:
DOI: https://doi.org/10.1023/A:1005648316531