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Enhanced production of laccase in Trametes vesicolor by the addition of ethanol

Abstract

In a medium containing 40 g ethanol l−1, laccase production by Trametes versicolor was 2.6 unit per ml of the supernatant, which was over 20 times higher than that without ethanol. Laccase activity with ethanol was quite comparable to that with the well-known inducers such as veratryl alcohol, xylidine and guaiacol. With other white-rot fungi, Coriolus hirsutus and Grifola frondosa, ethanol had a similar stimulatory effect on laccase production.

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References

  • Addleman K, Archibald F (1993) Kraft pulp bleaching and delignification by dikaryons and monokaryons of Trametes versicolor. Appl. Environ. Microbiol. 59: 266–273.

    Google Scholar 

  • Bradford MM (1984) A rapid and sensitive method for the quantitation of the microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Google Scholar 

  • Collins PJ, Kotterman MJJ, Field JA, Dobson ADW (1996) Oxidation of anthracene and benzo[a]prene by laccase from Trametes versicolor. Appl. Environ. Microbiol. 62: 4563–4567.

    Google Scholar 

  • Eggert C, Temp U, Eriksson KE (1996) The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase. Appl. Environ. Microbiol. 62: 1151–1158.

    Google Scholar 

  • Faure D, Bouillant ML, Bally R (1994) Isolation of Azospirillum lipoferum 4T Tn5 mutants affected in melanization and laccase activity. Appl. Environ. Microbiol. 60: 3413–3415.

    Google Scholar 

  • Field JA, Jong E, Feijoo-Costa G, Bont JAM (1993) Screening for ligninolytic fungi applicable to the biodegradation of xenobiotics. TIBTECH 11: 44–49.

    Google Scholar 

  • Fortina MG, Acquati A, Rossi P, Manachini PL, Di Gennaro C (1996) Production of laccase by Botrytis cinerea and fermentation studies with strain F226. J. Ind. Microbiol. 17: 69–72.

    Google Scholar 

  • Iimura Y, Hartikainen P, Tatsumi K (1996) Dechlorination of tetrachloroguaiacol by laccase of white-rot basidiomycete Coriolus versicolor. Appl. Microbiol. Biotechnol. 45: 434–439.

    Google Scholar 

  • Johannes C, Majcherczyk A, Huttermann A (1996) Degradation of anthracene by laccase of Trametes versicolor in the presence of different mediator compounds. Appl. Microbiol. Biotechnol. 46: 313–317.

    Google Scholar 

  • Mansur M, Suarez T, Fernandez-Larrea JB, Brizuela MA, Gonzalez AE (1997) Identification of a laccase gene family in the new lignin-degrading basidiomycete CECT 20197. Appl. Environ. Microbiol. 63: 2637–2646.

    Google Scholar 

  • Orth AB, Royse DJ, Tien M (1993) Ubiquity of lignin-degrading peroxidases among various wood-degrading fungi. Appl. Environ. Microbiol. 59: 4017–4023.

    Google Scholar 

  • Rescigno A, Sollai F, Curreli N, Rinaldi A, Sanjust E (1993) An extracellular laccase from Pleurotus sajor-caju. Ital. J. Biochem. 42: 227A-8A.

    Google Scholar 

  • Rodney PJ (1989) Biological principles for the effects of ethanol. Enz. Microbiol. Technol. 11: 130–153.

    Google Scholar 

  • Schliephake K, Lonergan GT (1996) Laccase variation during dye decolourisation in a 200-l packed-bed bioreactor. Biotechnol. Lett. 18: 881–886.

    Google Scholar 

  • Shin KS, Kim CJ (1998) Properties of laccase purified from nitrogen limited culture of white-rot fungus Coriolus hirsutus. Biotechnol. Tech. 12: 101–104.

    Google Scholar 

  • Skorobogatko OV, Stepanova EV, Gavrilova VP, Yaropolov AI (1996) Effects of inducers on the synthesis of extracellular laccase by Coriolus hirsutus, a basidial fungus. Prikl. Biokhim. Mikrobiol. 32: 545–548.

    Google Scholar 

  • Thurston CF (1994) The structure and function of fungal laccase. Microbiology 140: 19–26.

    Google Scholar 

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Lee, IY., Jung, KH., Lee, CH. et al. Enhanced production of laccase in Trametes vesicolor by the addition of ethanol. Biotechnology Letters 21, 965–968 (1999). https://doi.org/10.1023/A:1005646812381

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  • DOI: https://doi.org/10.1023/A:1005646812381

  • ethanol
  • laccase
  • Trametes versicolor