Abstract
Endo-polygalacturonase from Aspergillus ustus when immobilized on to modified silica gel retained 28% of its original activity. The immobilized enzyme could be re-used through 10 cycles of reaction with almost 90% retention of its original activity. It had increased thermostability over its soluble form: the half-life of the soluble enzyme at 40 °C was less than 10 h whereas the immobilized enzyme retained 82% of its activity after 10 h at 40 °C. Similarly, at 50 °C the half-life of the soluble enzyme was 30 min whereas that of the immobilized enzyme was 5 h.
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Narsimha Rao, M., Kembhavi, A. & Pant, A. Immobilization of endo-polygalacturonase from Aspergillus ustus on silica gel. Biotechnology Letters 22, 1557–1559 (2000). https://doi.org/10.1023/A:1005632917259
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DOI: https://doi.org/10.1023/A:1005632917259