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Cloning, analysis and expression of the gene for β-glycosidase of Thermus caldophilus GK24 and properties of the enzyme

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Abstract

The gene (bglT) encoding Thermus caldophilus GK24 β-glycosidase (Tca β-glycosidase) was cloned and sequenced. The gene contains an open reading frame encoding 431 amino acids with a M r of 48 658 Da. The bglT gene was expressed under the control of tac promoter on a high-copy plasmid in E. coli. The recombinant Tca β-glycosidase was purified 41.5-fold with a 59% yield and a specific activity of 83 U mg−1 protein.

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Authors and Affiliations

  1. Department of Genetic Engineering, Sungkyunkwan University, 300 Chunchon-Dong, Jangan-Ku, Suwon, 440-746, Korea

    Ki-Woong Han, Jinsang Yoo, Eun-Joo Oh, Jeong Jin Choi, Hyun-Kyu Kim & Suk-Tae Kwon

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  1. Ki-Woong Han
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  2. Jinsang Yoo
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  3. Eun-Joo Oh
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  4. Jeong Jin Choi
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  5. Hyun-Kyu Kim
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  6. Suk-Tae Kwon
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Correspondence to Suk-Tae Kwon.

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Han, KW., Yoo, J., Oh, EJ. et al. Cloning, analysis and expression of the gene for β-glycosidase of Thermus caldophilus GK24 and properties of the enzyme. Biotechnology Letters 23, 379–384 (2001). https://doi.org/10.1023/A:1005628106138

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