Abstract
An exogenous natural lung surfactant obtained from minced pig lungs can be produced by a technology using a low cost, DEAE-cellulose adsorbent. This surfactant is composed mainly with phospholipids and the two hydrophobic polypeptides, SP-B and SP-C, both of which are necessary for optimal function of surfactants used for treatment of respiratory distress syndrome.
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Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99.
Fujiwara T, Konishi M, Chida S, Okuyama K, Ogawa Y, Takeuchi Y, Nishida H, Kito H, Fujimura M, Nakamura H, Hashimoto T (1990) Surfactant replacement therapy with a single postventilatory dose of a reconstituted bovine surfactant in preterm neonates with respiratory distress syndrome-Final analysis of amulticenter, double-blind, randomized trial and comparison with similar trials. Pediatrics 86: 753-764.
Goerke J (1998) Pulmonary surfactant: Functions and molecular composition. Biochim. Biophys. Acta 1408: 79-89.
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
Robertson B (1988) Surfactant replacement therapy for severe neonatal respiratory distress syndrome-An international randomized clinical-trial. Pediatrics 82: 683-691.
Robertson B, Halliday HL (1998) Principles of surfactant replacement. Biochim. Biophys. Acta 1408: 346-361.
Robertson B, Curstedt T, Tubman R, Strayer D, Berggren P, Kok J, Koppe J, Vansonderen L, Halliday H, Mcclure G, Reid M, Oetemo SB, Okken A, Speer C, Schroter W, Svenningsen N, Walti H, Relier JP (1992) A 2-year follow-up babies enrolled in a European multicenter trial of porcine surfactant replacement for severe neonatal respiratory distress syndrome. Eur. J. Pediat. 151: 372-376.
Robertson B, Johansson J, Cursted T (2000) Synthetic surfactants to treat neonatal lung disease. Mol. Med. Today 6: 119-124.
Rouser G, Fleischer S, Yamamoto A (1970) Two dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5: 494-496.
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150: 76-85.
Soll RF (1998) Surfactant treatment of the very preterm infant. Biol. Neonate 74(Suppl 1): 35-42.
Spragg RG (1998) Surfactant therapy in acute respiratory distress syndrome. Biol. Neonate 74(Suppl 1): 15-20.
Veldhuizen R, Kaushik N, Orgeig S, Possmayer F (1998) The role of lipids in pulmonary surfactant. Biochim. Biophys. Acta 1408: 90-108.
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Kubrusly, F.S., de Lima Netto, S., Iourtov, D. et al. A natural surfactant from pig lungs. Biotechnology Letters 22, 1251–1253 (2000). https://doi.org/10.1023/A:1005625804225
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DOI: https://doi.org/10.1023/A:1005625804225