In vitro propagation of Catharanthus roseus was achieved using nodal explants. Bud induction was best on medium containing 1.0 mg benzyl aminopurine l−1. Hardening of rooted shoots to soil was very successful with 98% survival. Genetically transformed C. roseus plantlets were obtained after bombardment of nodal explants, which were then micropropagated, with DNA coated particles with green fluorescent protein (GFP) or β-glucuronidase (GUS) reporter genes. Histological studies showed that the gene insertion method proved effective with many cells and different tissues displaying the reporter gene signals, showing that gene expressions were rather stable.
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Banthorpe DV, Charlwood BV, Francis MJO (1972) The biosynthesis of monoterpenes. Chem. Rev. 72: 115–155.
Canel C, Lopes-Cardoso MI, Whitmer S, van der Fits L, Pasquali G, van der Heijden R, Hoge JHC, Verpoorte R (1998) Effects of overexpression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus. Planta 205: 414–419.
Chiu WL, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J (1996) Engineered GFP as a vital reporter in plants. Curr. Biol. 6: 325–330.
Choudhury S, Gupta K (1995) Studies on the germination mechanism of Catharanthus roseus (L.) G.Don cv. alba seeds: effects of temperature and promoters. Seed Sci. Technol. 23: 831–842.
Contin A, van der Heijden R, Lefeber AWM, Verpoorte R (1998) The iridoid glucoside secologanin is derived from the novel triose phosphate/pyruvate pathway in a Catharanthus roseus cell culture. FEBS Lett. 434: 413–416.
de Luca V, Marineau C, Brisson N (1989) Molecular cloning and analysis of cDNA encoding a plant tryptophan decarboxylase: Comparisson with animal dopa decarboxylase. Proc. Nat. Acad. Sci. USA 86: 2582–2586.
Firn RD (1986) Growth substance sensitivity: The need for clearer ideas, precise terms and purposeful experiments. Physiol. Plant 67: 267–272.
Hilliou F, Christou P, Leech MJ (1999) Development of an efficient transformation system for Catharanthus roseus cell cultures using particle bombardment. Plant Sci. 140: 179–188.
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901–3907.
Kaur K, Lodha P, Kant U (1996) In vitro regeneration of mosaic virus free Catharanthus roseus (L.) G. Don. plants through callus culture. J. Phytol. Res. 9: 25–28.
Klein TM, Gradziel T, Fromm MF, Sanford J (1988) Factors influencing gene delivery into Zea mays cells by high-velocity microprojectiles. Bio-Technol. 6: 559–563.
McKnight TD, Roessner CA, Devagupta R, Scott AI, Nessler CL (1990) Nucleotide sequence of a cDNA encoding the vacuolar protein strictosidine synthase from Catharanthus roseus. Nucl. Acids Res. 18: 4939–4939.
Meijer AH, de Waal A, Verpoorte R (1993) Purification of the cytochrome P450 enzyme geraniol-10-hydroxylase from cell cultures of Catharanthus roseus. J. Chromatogr. 635: 237–249.
Möllers C, Sarkar S (1989) Regeneration of healthy plants from Catharanthus roseus infected with mycoplasma-like organisms through callus culture. Plant Sci. 60: 83–89.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497.
van der Fits L, Memelink J (1997) Comparison of the activities of CaMV 35S and FMV 34S promoter derivatives in Catharanthus roseus cells transiently and stably transformed by particle bombardment. Plant Mol. Biol. 33: 943–946.
Verpoorte R, van der Heijden R, van Gulik WM, ten Hoopen HJG (1991) Plant biotechnology for the production of alkaloids: present status and prospects. In: Brossi A, ed. The Alkaloids, Vol. 40. San Diego: Academic Press, pp. 1–187.
Verpoorte R., van der Heijden R., Moreno P.R.H., (1997) Biosynthesis of terpenoid indole alkaloids in Catharanthus roseus cells. In: Cordell GA, ed. The Alkaloids, Vol. 49, San Diego: Academic Press, pp. 221–299.
Yun D-J, Hashimoto T, Yamada Y (1992) Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition. Proc. Nat. Acad. Sci. USA 89: 11799–11803.
Zárate R, Cantos M, Troncoso A (1997) Induction and development of adventitious shoots of Atropa baetica as a means of propagation. Euphytica 94: 361–366.
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Zárate, R., Memelink, J., van der Heijden, R. et al. Genetic transformation via particle bombardment of Catharanthus roseus plants through adventitious organogenesis of buds. Biotechnology Letters 21, 997–1002 (1999). https://doi.org/10.1023/A:1005622317333
- Catharanthus roseus
- genetic transformation
- green fluorescent protein
- particle bombardment